Links  |Sitemap  |  Contact  |  Home  |  中文   |  CAS
 HomeAbout UsResearchScientistsInt`l CooperationNews | Education & TrainingJoin UsPapersResources 
  New Papers
Multimedia
en20131028.jpg
The 5th International Conference on Rodent Biol...
Rodent adaptation and survival under global change
  Th...

Workshop on Phylogenetic comparative methods in...
The 31st IUBS GA and Conference on Biological ...
Special issue of Integrative Zoology: Biologica...
Exploring and integrating cellulolytic systems ...
      More>>
  Location: Home > New Papers
CRISPR-Cas9-Mediated Multiplex Gene Editing in CAR-T Cells
[ Update Time: 2016-12-14 ]

Chimeric antigen receptor (CAR) T cell therapy is a promising approach to treat cancer, such as B-cell malignancy1. However, the current standard treatment requires autologous adoptive cell transfer, which is expensive and time-consuming. For newborn and elder patients, it is often difficult to obtain enough T cells with good quality to generate patient-specific CAR-T cells. To make CAR-T therapy more accessible, it is highly desirable to develop an allogeneic adoptive transfer strategy, in which universal CAR-T cells derived from T cells from healthy donors can be applied to treat multiple patients. For this strategy to work, the αβ T-cell receptor (TCR) on allogeneic CAR-T cells needs to be eliminated to avoid graft-versus-host-disease (GVHD), and human leukocyte antigens class I (HLA-Is) on CAR-T cells need to be removed to minimize their immunogenicity. Previous studies have shown that mutation in TCRα subunit constant (TRAC) leads to loss of αβ TCR on T-cell surface2, and beta-2 microglobulin (B2M) is essential for cell-surface expression of HLA-I heterodimers3. Thus, we attempted to target TRAC and B2M genes in CAR-T cells. Considering blocking programmed death-1 (PD-1) signaling can effectively treat cancers via reversing immunosuppression, we also targeted PD-1 in CAR-T cells to render them nonresponsive to PD-1 signaling4...

http://www.nature.com/cr/journal/vaop/ncurrent/full/cr2016142a.html 

 
Copyright 1995-2020 INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES
Tel: +86-10-64807098, Fax: +86-10-64807099, Email: ioz@ioz.ac.cn
Address: 1 Beichen West Road, Chaoyang District, Beijing 100101, P.R.China
Internet Explorer 6.0+, best view with resolution 1024x768