Links  |Sitemap  |  Contact  |  Home  |  中文   |  CAS
 HomeAbout UsResearchScientistsInt`l CooperationNews | Education & TrainingJoin UsPapersResources 
  Location: Home > Papers
  Papers
Title: Lipid-mediated phase separation of AGO proteins on the ER controls nascent-peptide ubiquitination
Author: Gao, Yajie; Zhu, Yuanxiang; Wang, Hailong; Cheng, Ying; Zhao, Dongbo; Sun, Qinmiao; Chen, Dahua
Abstract: AGO/miRNA-mediated gene silencing and ubiquitin-mediated protein quality control represent two fundamental mechanisms that control proper gene expression. Here, we unexpectedly discover that fly and human AGO proteins, which are key components in the miRNA pathway, undergo lipid-mediated phase separation and condense into RNP granules on the endoplasmic reticulum (ER) membrane to control protein production. Phase separation on the ER is mediated by electrostatic interactions between a conserved lipid-binding motif within the AGOs and the lipid PI(4,5)P-2. The ER-localized AGO condensates recruit the E3 ubiquitin ligase Ltn1 to catalyze nascent-peptide ubiquitination and coordinate with the VCP-Ufd1-Npl4 complex to process unwanted protein products for proteasomal degradation. Collectively, our study provides insight into the understanding of post-transcription-translation coupling controlled by AGOs via lipid-mediated phase separation.
Corresponding author: Qinmiao Sun
Subject:
Impact Factor: 19.328
Authors units:
PubYear: 2022
Volume: 82
Issue: 7
Page: 1313-
Journal: Molecular Cell
The full text link:
ISSN:
URL:
   

CLOSE

 
Copyright 1995-2020 INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES
Tel: +86-10-64807098, Fax: +86-10-64807099, Email: ioz@ioz.ac.cn
Address: 1 Beichen West Road, Chaoyang District, Beijing 100101, P.R.China
Internet Explorer 6.0+, best view with resolution 1024x768