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Name:
 Xiang-dong Li
Subject:
 Cell biology
Tel/Fax:
 +86-10-64806015  / 
E-mail:
 lixd@ioz.ac.cn
Address:
 The State Key Laboratory of Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences 1 Beichen West Road, Chaoyang District, Beijing 100101, P.R.China
More:
 Group of Cell Motility and Muscle Contraction      
Resume:

1990-1993, Institute of Entomology, Chinese Academy of Sciences, M.S. student
1993-1996, Institute of Biochemistry, Chinese Academy of Sciences, Ph.D student
1996-2004, Department of Physiology, University of Massachusetts Medical School, postdoctoral fellow
2004-2008, Department of Physiology, University of Massachusetts Medical School, instructor and research assistant professor
2008-2009, Department of Cell Biology, University of Massachusetts Medical School, research assistant professor
2009-, Institute of Zoology, Chinese Academy of Sciences, professor

Research Interests:

1) Insect muscle differentiation

2) Cell motility and intracellular trafficking

3) Myosin-targeted drug development

Awards and Honors:

Professional Activities:

Research Grants:

Selected Publications:

(*corresponding author):

  1. Rui Zhou, Jiabin Pan, Wen-Bo Zhang, and Xiang-dong Li* (2024) Myosin-5a facilitates stress granule formation by interacting with G3BP1. Cellular and Molecular Life Sciences 81:430.
  2. Jiabin Pan, Rui Zhou, Lin-Lin Yao, Jie Zhang, Ning Zhang, Qin-Juan Cao, Shaopeng Sun, and Xiang-dong Li* (2024) Identification of a third myosin-5a-melanophilin interaction that mediates the association of myosin-5a with melanosomes. eLife 13:RP93662.
  3. Jie Hao, Chang Liu, Ning Zhang, Jing Li, Tong Ni, Mingbo Qu, and Xiang-dong Li* (2024) Alternative relay regulates the adenosine triphosphatase activity of Locusta migratoria striated muscle myosin. Insect Science 31:435-447.
  4. Qing-Juan Cao, Ning Zhang, Rui Zhou, Lin-Lin Yao, and Xiang-dong Li* (2019) The cargo adaptor proteins RILPL2 and melanophilin co-regulate myosin-5a motor activity. Journal of Biological Chemistry 294 (29): 11333–11341.
  5. Mei Shen, Ning Zhang, Sanduo Zheng, Wen-Bo Zhang, Hai-Man Zhang, Zekuan Lu, Qian Peter Su, Yujie Sun, Keqiong Ye, and Xiang-dong Li* (2016) Calmodulin in complex with the first IQ motif of myosin-5a functions as an intact calcium sensor. Proceedings of the National Academy of Sciences USA 113: E5812-E5820.
  6. Wen-Bo Zhang, Lin-Lin Yao, and Xiang-dong Li* (2016) The Globular Tail Domain of Myosin-5a Functions As a Dimer in Regulating the Motor Activity. Journal of Biological Chemistry 291: 13571-13579.
  7. Zekuan Lu, Xiao-Nan Ma, Hai-Man Zhang, Huan-Hong Ji, Hao Ding, Jie Zhang, Dan Luo, Yujie Sun, and Xiang-dong Li* (2014) Mouse Myosin-19 is a Plus-end-directed, High-duty Ratio Molecular Motor. Journal of Biological Chemistry 289: 18535-18548.
  8. Zekuan Lu, Mei Shen, Yang Cao, Hai-Man Zhang, Lin-Lin Yao, Xiang-dong Li* (2012) Calmodulin bound to the first IQ motif is responsible for calcium-dependent regulation of myosin 5a. Journal of Biological Chemistry 287: 16530-16540.
  9. Xiang-dong Li*, Hyun Suk Jung, Qizhi Wang, Reiko Ikebe, Roger Craig, and Mitsuo Ikebe (2008) The globular tail domain puts on the brake to stop the ATPase cycle of the myosin Va. Proceedings of the National Academy of Sciences USA 105: 1140-1145.
  10. Xiang-dong Li*, Hyun Suk Jung, Katsuhide Mabuchi, Roger Craig, and Mitsuo Ikebe (2006) The globular tail domain of myosin Va functions as an inhibitor of the myosin Va motor. Journal of Biological Chemistry 281:21789-21798.

Publication list (*corresponding author; #, equal contribution):

  1. Xiao-Xiao Zhu, Qin Tian, Ning Zhang, Qin-Rong Tan, Guangfei Tang, Zhang Lei, Bing Chen, and Xiang-dong Li* (2025) Characterization of Candida albicans myosin-1 motor function and its regulation by phosphorylation at TEDS site. International Journal of Biological Macromolecules 307: 142314 (https://doi.org/10.1016/j.ijbiomac.2025.142314).
  2. Shaopeng Sun, Yi-Ning Lu, and Xiang-dong Li* (2025) Structure of the Inhibited Smooth Muscle Myosin and Its Implications on the Regulation of Insect Striated Muscle Myosin. Life 15(3): 379 (https://doi.org/10.3390/life15030379).
  3. Di Zhang, Xiao-Hua Song, Dan Yang, Mu-Zi Ge, Jun Qiu, Han-Qing Jiang, Yan-Yan Sun, Xiang-dong Li* and Yi-JunWu* (2025) Subchronic Exposure to Low-Dose Chlorfenapyr and Emamectin Benzoate Disrupts Kidney Metabolism in Rats. Toxics 13(1): 65 (https://doi.org/10.3390/toxics13010065).
  4. Rui Zhou, Jiabin Pan, and Xiang-dong Li* (2025) Determination of Dissociation Constants for the Interaction of Myosin-5a with its Cargo Protein Using Microscale Thermophoresis (MST). Bio-Protocol 15:1364 (doi: 10.21769/bioprotoc.5176).
  5. Rui Zhou, Jiabin Pan, Wen-Bo Zhang, and Xiang-dong Li* (2024) Myosin-5a facilitates stress granule formation by interacting with G3BP1. Cellular and Molecular Life Sciences 81:430 (doi: 10.1007/s00018-024-05468-w).
  6. Jiabin Pan, Rui Zhou, Lin-Lin Yao, Jie Zhang, Ning Zhang, Qin-Juan Cao, Shaopeng Sun, and Xiang-dong Li* (2024) Identification of a third myosin-5a-melanophilin interaction that mediates the association of myosin-5a with melanosomes. eLife 13:RP93662 (https://doi.org/10.7554/eLife.93662.1).
  7. Miao Wei, Ning Zhang and Xiang-dong Li* (2024) Characterisation of the myosin light chain kinase (MLCK) gene of Locusta migratoria and the encoded MLCK. Insect Molecular Biology 33:338-349 (doi: 10.1111/imb.12902). 
  8. Lin-Lin Yao, Wei-Dong Hou, Yi Liang, Xiang-dong Li, Huan-Hong Ji* (2024). Spire2 and Rab11a synergistically activate myosin-5b motor function. Biochemical and Biophysical Research Communications 703:149653
  9. Qiaowan Chen, Chang Liu, Hao Qi, Ningjie Wu, Zunyong Liu, Qin Tian, Xiao‑Xiao Zhu, Xiang-dong Li, Yun Chen and Zhonghua Ma (2024) A novel highly antifungal compound ZJS‑178 targeting myosin I inhibits the endocytosis and mycotoxin biosynthesis of Fusarium graminearum. Crop Health 2:14 (https://doi.org/10.1007/s44297-024-00034-z).
  10. Min Wang, Xinxin Li, Chongnv Wang, Ming Zou, Jing Yang, Xiang-Dong Li, Baocheng Guo (2024) Asymmetric and parallel subgenome selection co-shape common carp domestication. BMC Biology 22:4 (doi: 10.1186/s12915-023-01806-9).
  11. Jie Hao, Chang Liu, Ning Zhang, Jing Li, Tong Ni, Mingbo Qu, and Xiang-dong Li* (2024) Alternative relay regulates the adenosine triphosphatase activity of Locusta migratoria striated muscle myosin. Insect Science 31:435–447.
  12. Qin-Rong Tang and Xiang-dong Li* (2023) Fungal myosin-1 homologs as key molecular motors at the membrane-cytoskeleton interface. Fungal Biology Reviews 45:100318.
  13. Hui-Fang Liang and Xiang-dong Li* (2022) Locusta migratoria flight muscle troponin partially activates thin filament in a calcium-dependent manner. Insect Molecular Biology 31:346–355.
  14. Ning Zhang#, Shu-Jie Zhou#, Huan-Hong Ji, and Xiang-dong Li* (2022) Effects of the IQ1 motif of Drosophila myosin-5 on the calcium interaction of calmodulin. Cell Calcium 103: 102549.
  15. Tong Ni#, Min Yuan#, Huan-Hong Ji, Guangfei Tang, Yun Chen, Zhonghua Ma, and Xiang-dong Li* (2020) Effects of Mutations in the Phenamacril-Binding Site of Fusarium Myosin‑1 on Its Motor Function and Phenamacril Sensitivity. ACS Omega 5: 21815−21823.
  16. Hui-Fang Liang, Jing Li, and Xiang-dong Li* (2020) Identification and characterization of troponin genes in Locusta migratoria. Insect Molecular Biology 29(4): 391-403.
  17. Qing-Juan Cao#, Ning Zhang#, Rui Zhou, Lin-Lin Yao, and Xiang-dong Li* (2019) The cargo adaptor proteins RILPL2 and melanophilin co-regulate myosin-5a motor activity. Journal of Biological Chemistry 294 (29): 11333–11341.
  18. Huan-Hong Ji#, Lin-Lin Yao#, Chang Liu, and Xiang-dong Li* (2019) Regulation of Myosin-5b by Rab11a and the Rab11 family interacting protein 2. Bioscience Reports 39 (1): BSR20181252.
  19. Ning Zhang#, Lin-Lin Yao#, and Xiang-dong Li* (2018) Regulation of Class V Myosin. Cellular and Molecular Life Science 75(2): 261-273.
  20. Hai-Man Zhang, Huan-Hong Ji, Tong Ni, Rong-Na Ma, Aibing Wang, and Xiang-dong Li* (2017) Characterization of Blebbistatin Inhibition of Smooth Muscle Myosin and Nonmuscle Myosin-2. Biochemistry 56(32):4235-4243.
  21. Ning Zhang and Xiang-dong Li* (2017) Directional Transportation of Assembled Molecular Linear Motors, in Supramolecular Chemistry of Biomimetic Systems (Li, J., Ed.) Springer Nature, Singapore.
  22. Mei Shen#, Ning Zhang#, Sanduo Zheng#, Wen-Bo Zhang, Hai-Man Zhang, Zekuan Lu, Qian Peter Su, Yujie Sun, Keqiong Ye, and Xiang-dong Li* (2016) Calmodulin in complex with the first IQ motif of myosin-5a functions as an intact calcium sensor. Proceedings of the National Academy of Sciences USA 113: E5812-E5820.
  23. Jing Li, Zekuan Lu, Jing He, Qianquan Chen, Xianhui Wang, Le Kang, and Xiang-dong Li* (2016) Alternative Exon-encoding Regions of Locusta migratoria Muscle Myosin Modulate the pH dependence of ATPase Activity. Insect Molecular Biology 25:689-700.
  24. Nan Chen, Yong-Liang Fan*, Yu Bai, Xiang-dong Li, Zhan-Feng Zhang, and Tong-Xian Liu* (2016) Cytochrome P450 gene, CYP4G15, modulates hydrocarbon production in the pea aphid, Acyrthosiphon pisum. Insect Biochemistry and Molecular Biology 76: 84-94.
  25. Lin-Lin Yao, Mei Shen, Zekuan Lu, Mitsuo Ikebe, and Xiang-dong Li* (2016) Identification of the isoform-specific interactions between the tail and the head of class V myosin. Journal of Biological Chemistry 291: 8241-8250.
  26. Wen-Bo Zhang, Lin-Lin Yao, and Xiang-dong Li* (2016) The Globular Tail Domain of Myosin-5a Functions As a Dimer in Regulating the Motor Activity Journal of Biological Chemistry 291: 13571 -13579.
  27. Hanna Witwick, Hong Jia, Artem Kutikov, Pablo Reyes-Gutierrez, Xiang-dong Li, Paul R. Odgren* (2015) TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast Acidification and Resorption. PLOS ONE 10: e0127537
  28. Chengqi Zhang#, Yun Chen#, Yanni Yin, Huan-Hong Ji, Won-Bo Shim, Yiping Hou, Mingguo Zhou*, Xiang-dong Li*, and Zhonghua Ma* (2015) A small molecule species-specifically inhibits Fusarium myosin I. Environmental Microbiology 17(8): 2735–2746.
  29. Huan-Hong Ji, Hai-Man Zhang, Mei Shen, Lin-Lin Yao, Xiang-dong Li* (2015) The motor function of Drosophila melanogaster myosin-5 is activated by calcium and cargo-binding protein dRab11. Biochemical Journal 469 (1):135-144.
  30. Lin-Lin Yao#, Qing-Juan Cao#, Hai-Man Zhang#, Jie Zhang, Yang Cao, and Xiang-dong Li* (2015) Melanophilin Stimulates Myosin-5a Motor Function by Allosterically Inhibiting the Interaction between the Head and Tail of Myosin-5a. Scientific Reports 5:10874.
  31. Zekuan Lu, Xiao-Nan Ma, Hai-Man Zhang, Huan-Hong Ji, Hao Ding, Jie Zhang, Dan Luo, Yujie Sun, and Xiang-dong Li* (2014) Mouse Myosin-19 is a Plus-end-directed, High-duty Ratio Molecular Motor. Journal of Biological Chemistry 289: 18535–18548.
  32. Yang Cao, Howard D. White, and Xiang-dong Li* (2014) Drosophila myosin-xx functions as an actin-binding protein to facilitate the interaction between zyx102 and actin. Biochemistry 53, 350–360.
  33. Rong-Na Ma, Katsuhide Mabuchi, Jing Li, Zekuan Lu, Chih-Lueh Albert Wang, and Xiang-dong Li* (2013) Cooperation between the Two Heads of Smooth Muscle Myosin Is Essential for Full Activation of the Motor Function by Phosphorylation. Biochemistry 52, 6240−6248.
  34. Zekuan Lu#, Mei Shen#, Yang Cao, Hai-Man Zhang, Lin-Lin Yao, Xiang-dong Li* (2012) Calmodulin bound to the first IQ motif is responsible for calcium-dependent regulation of myosin 5a. Journal of Biological Chemistry 287: 16530-16540.
  35. 曹洋，沈梅，张洁，李向东* (2011) 果蝇非常规肌球蛋白的结构与功能。应用昆虫学报 48: 239-246.
  36. Nobuhisa Umeki, Hyun Suk Jung, Shinya Watanabe, Tsuyoshi Sakai, Xiang-dong Li, Reiko Ikebe, Roger Craig, and Mitsuo Ikebe* (2009）The tail binds to the head-neck domain, inhibiting ATPase activity of myosin VIIA Proceedings of the National Academy of Sciences USA 106:8483-8.
  37. Xiang-dong Li*, Hyun Suk Jung, Qizhi Wang, Reiko Ikebe, Roger Craig, and Mitsuo Ikebe (2008) The globular tail domain puts on the brake to stop the ATPase cycle of the myosin Va. Proceedings of the National Academy of Sciences USA 105: 1140-1145 (Citied in “Faculty of 1000”).
  38. Zhiping Wang, Jeffrey G. Edwards, Nathan Riley, D. William Provance, Jr., Ryan Karcher, Xiang-dong Li, Ian G. Davison, Mitsuo Ikebe, John A. Mercer, Julie A. Kauer, and Michael D. Ehlers* (2008) Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity. Cell 135, 535-548.
  39. Osamu Sato, Xiang-dong Li, and Mitsuo Ikebe* (2007) Myosin Va becomes a low duty ratio motor in the inhibited form. Journal of Biological Chemistry 282:13228-13239.
  40. Xiang-dong Li*, Hyun Suk Jung, Katsuhide Mabuchi, Roger Craig, and Mitsuo Ikebe (2006) The globular tail domain of myosin Va functions as an inhibitor of the myosin Va motor. Journal of Biological Chemistry 281:21789-21798 (Citied in “Faculty of 1000”).
  41. Xiang-dong Li, Reiko Ikebe, and Mitsuo Ikebe* (2005) Activation of Myosin Va function by melanophilin, a specific docking partner of Myosin Va. Journal of Biological Chemistry 278: 29435-29441.
  42. Xiang-dong Li, Katsuhide Mabuchi, Reiko Ikebe, and Mitsuo Ikebe* (2004) Ca2+ induced activation of ATPase activity of myosin Va is accompanied with a large conformational change. Biochemical and Biophysical Research Communications 315: 538-545.
  43. Xiang-dong Li and Mitsuo Ikebe* (2003) Two functional heads are required for full activation of smooth muscle myosin. Journal of Biological Chemistry 278: 29435-29441.
  44. Yu Chen, Norio Takizawa, Jessica L Crowley, Sang W. Oh, Cheryl L. Gatto, Taketoshi Kambara, Osamu Sato, Xiang-dong Li, Mitsuo Ikebe, and Elizabeth J. Luna* (2003) F-actin and myosin II binding domains in supervillin. Journal of Biological Chemistry  278:46094-46106
  45. Xin Zhou, Xiang-dong Li, Jian-zhong Yuan, Zhen-hua Tang, and Wang-yi Li* (2000) Toxicity of Cinnamomin-A new type II ribosome-inactivating protein to ballworm and mosquito. Insect Biochemistry and Molecular Biology 30(3): 259-264.
  46. Xiang-dong Li, Junya Saito, Reiko Ikebe, Katsuhide Mabuchi, and Mitsuo Ikebe* (2000) The interaction between the regulatory light chain domains on two heads is critical for regulation of smooth muscle myosin. Biochemistry 39(9): 2254-2260.
  47. Xiang-dong Li, Troy E. Rhodes, Reiko Ikebe, Taketoshi Kambara, Howard D. White and Mitsuo Ikebe* (1998) Effects of mutations in the γ-phosphate binding site of myosin on its motor function. Journal of Biological Chemistry 273(42): 27404-27411.
  48. Wang-yi Liu*, Huai-yang Chen, Wen-feng Chen, Xiang-dong Li, Norbert Ulbrich and Werner Schroder (1997) A proposed position of a disulfide bridge in the molecules of cinnamomin and porrectin-two new type II ribosome-inactivating proteins isolated from the seeds of camphor trees. Biochemistry and Molecular Biology International 44(5): 1045-1049.
  49. Xiang-dong Li, Wen-feng Chen, Wang-yi Liu*, and Gui-hai Wang (1997) Large-scale purification of two new ribosome-inactivating proteins --- cinnamomin and camphorin from the seed of Cinnamomum camphora. Protein Expression and Purification 10:27-31.
  50. 李向东，刘望夷* (1997) 核糖体失活蛋白研究进展。生命的化学17(2): 15-18.
  51. 李向东，刘望夷* (1997) 核糖体失活蛋白结构功能与分布。细胞生物学杂志19(2): 69-75.
  52. Xiang-dong Li, Wang-yi Liu*, and Ching-i Niu. (1996) Purification of a new ribosome-inactivating protein from the seeds of Cinnamomum porrectum and characterization of the RNA N-glycosidase activity of the toxic protein. Biological Chemistry, 377: 825-831.
  53. Jun Ling, Xiang-dong Li, Xiao-hua Wu, and Wang-yi Liu* (1995) Topological requirements for recognition and cleavage of DNA by ribosome-inactivating proteins. Biological Chemistry Hoppe-Seyler, 376: 637-641.
  54. 李向东， 唐振华* (1993) 马拉硫磷羧酸酯酶在小菜蛾抗药性中的作用。昆虫学研究集刊11: 1-10.