Location: Home > Scientists
  Scientists
Name:
 CUI Feng
Subject:
 Zoology
Tel/Fax:
 +86-10-64807218  /  +86-10-64807099
E-mail:
 cuif@ioz.ac.cn
Address:
 Bei Chen Xi Lu 1-5, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
More:
 Genomics of vector insect and virus      
Resume:

1. Education

  • Sept. 2003-July 2006, Ph.D. in Zoology, Genetics of insecticide resistance in insects, Institute of Zoology, CAS, Beijing, China
  • Sept. 1999-July 2002, Master in Agronomy, Insect physiology, Shandong Agricultural University, Tai’an, China
  • Sept. 1995-July 1999, Bachelor in Agronomy, Forestry, Shandong Agricultural University, Tai’an, China

2. Work Experience

  • Jan. 2017 - present, Professor, Institute of Zoology, CAS
  • Jan. 2016 - Dec. 2016: Innovative Professor, Institute of Zoology, CAS
  • Jan. 2009 - Dec. 2015: Associate Professor, Institute of Zoology, CAS
  • July 2006 - Dec. 2008: Assistant Professor, Institute of Zoology, CAS
  • July 2007 - Dec. 2008: Associate Research, Department of Biochemistry, Kansas State University

3. Membership of Societies

  • Council Member and Deputy Secretary General, Entomological Society of China, 2022-2027
  • Deputy Director, Committee of Insect Comparative Immunology and Interactions, Entomological Society of China, 2022-2027

4. Editorial Board Members

  • Current Opinion in Insect Science, 2023-present
  • Insect Science, 2023-present
  • Journal of Insect Physiology, 2022-present
  • Insects, 2021-present
  • Microbiology Spectrum, 2021-present
  • Acta Entomologica Sinica, 2021-present

5. Research directions and achievements

As leader of Genomics of Vector Insect and Virus Group, my main research direction is exploring the molecular mechanisms of virus transmission from the interaction among insect vector, virus, and host plant in the model of small brown planthopper Laodelphax striatellus and rice stripe virus (RSV). This includes: entry and release mechanisms of virus in salivary glands and gut of insect vector; immune interaction mechanisms of insect vector and virus; functions of saliva effectors in viral transmission and the adaptation of insect vector to host plant; and genomic adaptation of virus during the transmission between insect vector and host plant. Another direction aims to explore viromes in mosquitoes and the infectious risk to human and the underlying molecular mechanisms.

Several important findings are as follows:

(1) Importin α2 and Flotillin 2 are key cofactors exploited by RSV to enter salivary glands and guts of insect vectors, respectively (PNAS 2021; Journal of Virology 2022).

(2) Exosomes facilitate RSV to overcome the salivary gland barrier for viral transmission from insect vectors to host plants with the aid of exosomal protein exportin 6 (PNAS 2022).

(3) RSV uniquely induces a long-winged morph in male insect vector through Akt-Encounter-insulin growth factor signaling pathway to spread the virus over long distances (PNAS 2024).

(4) Rice Flotillin 1 is a plasmodesmata (PD)-associated protein and regulates the PD pore sizes to facilitate the cell-to-cell transport of RSV and other rice viruses (Plant Biotechnology Journal 2024).

(5) Nuclear trafficking of RSV inhibits viral replication in insect vector through inducing apoptotic reaction (Protein & Cell 2022); RSV ensures viral stability in the hemolymph of insect vector through suppressing PPO activation (mBio 2020); JNK pathway is activated by RSV to induce Flotillin 2 expression for viral replication (eLife 2017; Journal of Virology 2022; Insect Biochemistry and Molecular Biology 2023).

(6) The 3'-termini of RSV genomes vary in the rotation between insect vector and host plant (New Phytologist 2018); the terminal variation of the viral genome controls RSV replication in insect vectors with the coordination of insect endogenous microRNAs and virus-derived small RNAs (PLoS Pathogens 2021, 2022).

(7) Species-specific viromes in a relatively small area are limited by viral interspecific competition and food sources, whereas the viromes of mosquito species in large geographic regions may be governed by ecological interactions between mosquitoes and local environmental factors (Microbiome 2023).

Research Interests:

Awards and Honors:

Professional Activities:

Research Grants:

Selected Publications:

* corresponding author # co-first author

  1. Yu J#, Zhao W#, Chen X, Lu H, Xiao Y, Li Q, Luo L, Kang L*, Cui F*. 2024. A plant virus manipulates the long-winged morph of insect vectors. Proc. Natl. Acad. Sci. U.S.A. 121: e2315341121.
  2. Cheng X#, Zhao W#*, Liang G, Lu H, Fu Y, Li Y, Cui F*. 2024. Construction of cytomegalovirus promoter-driven gene expression system in Laodelphax striatellus. Insect Science. 31: 720-732.
  3. Wu J#, Zhang Y#, Li F#, Zhang X#, Ye J, Wei T, Li Z, Tao X, Cui F, Wang X, Zhang L, Yan F, Li S, Liu Y, Li D, Zhou X*, Li Y*. 2024. Plant virology in the 21st century in china: recent advances and future directions. J Integr Plant Biol. 66: 579–622.
  4. Ge P#, Lu H#, Wang W, Ma Y, Li Y, Zhou T, Wei T, Wu J, Cui F*. 2024 Plasmodesmata-associated Flotillin positively regulates broad-spectrum virus cell-to-cell trafficking. Plant Biotechnology Journal. 22: 1387-1401.
  5. Gong Q#, Wang Y#, He L, Huang F, Zhang D, Wang Y, Wei X, Han M, Deng H, Luo L, Cui F, Hong Y, Liu Y*. 2023. Molecular basis of methyl-salicylate- mediated plant airborne defence. Nature. 622:139-148.
  6. Liu Q#, Cui F#, Liu X, Fu Y, Fang W, Kang X, Lu H, Li S, Liu B, Guo W, Xia Q*, Kang L*, Jiang F*. 2023. Association of virome dynamics with mosquito species and environmental factors. Microbiome. 11: 101.
  7. Zhao W#, Wang W#, Xiao Y, Cui F*. 2023. c-Jun regulates flotillin 2 transcription to benefit viral accumulation in insect vectors. Insect Biochemistry and Molecular Biology. 152: 103894.
  8. Lu H#, Zhu J#, Yu J, Li Q, Luo L, Cui F*. 2022. Key role of exportin 6 in exosome-mediated viral transmission from insect vectors to plants. Proc. Natl. Acad. Sci. U.S.A. 119: e2207848119.
  9. Zhao W, Li Q, Sun M, Xiao Y, Cui F*. 2022. Interaction between endogenous microRNAs and virus-derived small RNAs controls viral replication in insect vectors. PLoS Pathogens. 18: e1010709.
  10. Tong L#, Chen X#, Wang W#, Xiao Y, Yu J, Lu H, Cui F*. 2022. Alternative splicing landscape of small brown planthopper and different response of JNK2 isoforms to rice stripe virus infection. Journal of Virology. 96: e0171521.
  11. Lu H, Li J, Yang P, Jiang F, Liu H, Cui F*. 2022. Mutation in the RNA-dependent RNA polymerase of a symbiotic virus is associated with the adaptability of the viral host. Frontiers in Microbiology. 13: 883436.
  12. Wang W#, Qiao L#, Lu H, Chen X, Wang X, Yu J, Zhu J, Xiao Y, Ma Y, Wu Y, Zhao W, Cui F*. 2022. Flotillin 2 facilitates the infection of a plant virus in the gut of insect vector. Journal of Virology. 96: e0214021.
  13. Zhao W#, Zhu JJ#, Lu H, Zhu JM, Jiang F, Wang W, Luo L, Kang L, Cui F*. 2022. The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication. Protein & Cell. 13: 360-378.
  14. Ma Y#, Lu H#, Wang W, Zhu J, Zhao Wan, Cui F*. 2021. Membrane association of importin α facilitates viral entry into salivary gland cells of vector insects. Proc. Natl. Acad. Sci. U.S.A. 118 (30): e2103393118
  15. Zhao W, Yu J, Jiang F, Wang W, Kang L, Cui F*. 2021. Coordination between terminal variation of the viral genome and insect microRNAs regulates rice stripe virus replication in insect vectors. PLoS Pathogens. 17: e1009424.
  16. Xiao Y#, Li Q#, Wang W, Fu Y, Cui F*. 2021. Regulation of RNA interference pathways in the insect vector Laodelphax striatellus by viral proteins of rice stripe virus. Viruses. 13: 1591.
  17. Zhu J, Eid FE, Tong L, Zhao W, Wang W, Heath LS, Kang L, Cui F*. 2021. Characterization of protein-protein interactions between rice viruses and vector insects. Insect Science. 28: 976-986.
  18. Chen X, Yu J, Wang W, Lu H, Kang L*, Cui F*. 2020. A plant virus ensures viral stability in the hemolymph of vector insects through suppressing prophenoloxidase activation. mBio. 11: e01453-20.
  19. Zhao W#, Li Q#, Cui F*. 2020. Potential functional pathways of plant RNA virus-derived small RNAs in a vector insect. Methods. 183: 38-42.
  20. Lu H, Zhu J, Yu J, Chen X, Kang L*, Cui F*. 2020. A symbiotic virus facilitates aphid adaptation to host plants by suppressing jasmonic acid responses. Molecular Plant-Microbe Interactions. 33: 55-65.
  21. Li S, Jiang F, Lu H, Kang X, Wang Y, Zou Z, Wen D, Zheng A, Liu C, Liu Q, Kang L, Xia Q*, Cui F*. 2020. Mosquito diversity and population genetic structure of six mosquito species from Hainan Island. Frontiers in Genetics. 11: 602863.
  22. Zhao W#, Wang Q#, Xu Z, Liu R, Cui F*. 2019. Distinct replication and gene expression strategies of the Rice stripe virus in vector insects and host plants. Journal of General Virology. 100: 877-888.
  23. Zhao W#, Wang Q#, Xu Z#, Liu R, Cui F*. 2019. Immune responses induced by different genotypes of the disease-specific protein of Rice stripe virus in the vector insect. Virology. 527: 122-131.
  24. Wang X#, Wang W#, Zhang W, Li J, Cui F*, Qiao L*. 2019. Immune function of an angiotensin-converting enzyme against Rice stripe virus infection in a vector insect. Virology. 533: 137-144.
  25. Li J#, Zhao W#, Wang W, Zhang L, Cui F*. 2019. Evaluation of Rice stripe virus transmission efficiency by quantification of viral load in the saliva of insect vector. Pest Management Science. 75: 1979-1985.
  26. Cui N#, Lu H#, Wang T, Zhang W, Kang L*, Cui F*. 2019. Armet, an aphid effector protein, induces pathogen resistance in plants by promoting the accumulation of salicylic acid. Philosophical Transactions of the Royal Society B-Biological Sciences. 374: 20180314.
  27. Zhao W#, Xu Z#, Zhang X, Yang M, Kang L*, Liu R*, Cui F*. 2018. Genomic variations in the 3'-termini of Rice stripe virus in the rotation between vector insect and host plant. New Phytologist. 219: 1085-1096.
  28. Yang M#, Xu Z#, Zhao W#, Liu Q, Li Q, Lu L, Liu R*, Zhang X*, Cui F*. 2018. Rice stripe virus-derived siRNAs play different regulatory roles in rice and in the insect vector Laodelphax striatellus. BMC Plant Biology. 18: 219.
  29. Zhu J#, Jiang F#, Wang X, Yang P, Bao Y, Zhao W, Wang W, Lu H, Wang Q, Cui N, Li J, Chen X, Luo L, Yu J, Kang L*, Cui F*. 2017. Genome sequence of the small brown planthopper Laodelphax striatellus. GigaScience, 6:1-12.
  30. Wang W#, Zhao W#, Li J#, Luo L, Kang L*, Cui F*. 2017. The c-Jun N-terminal kinase pathway of a vector insect is activated by virus capsid protein and promotes viral replication. eLife. 6: e26591.
  31. Zhang L#, Lu H#, Guo K#, Yao S, Cui F*. 2017. Insecticide resistance status and detoxification enzymes of wheat aphids Sitobion avenae and Rhopalosiphum padi. Science China Life Sciences. 60, 927-930.
  32. Guo K, Yang P, Chen J, Lu H, Cui F*. 2017. Transcriptomic responses of three aphid species to chemical insecticide stress. Science China Life Sciences. 60, 931-934.
  33. Cui N#, Yang P#, Guo K, Kang L*, Cui F*. 2017. Large-scale gene expression reveals different adaptations of Hyalopterus persikonus to winter and summer host plants. Insect Science, 24, 431-442.
  34. Zhao W, Yang P, Kang L*, Cui F*. 2016. Different pathogenicities of rice stripe virus from the insect vector and from viruliferous plants. New Phytologist. 210: 196-207.
  35. Zhao W, Lu L, Yang P, Cui N, Kang L*, Cui F*. 2016. Organ-specific transcriptome response of the small brown planthopper toward rice stripe virus. Insect Biochemistry and Molecular Biology, 70: 60-72.
  36. Cui F#, Zhao W#, Luo L, Kang L. 2016. Rice responses and resistance to planthopper-borned viruses at transcriptomic and proteomic levels. Current Issues in Molecular Biology. 19: 43-52.
  37. Lu H, Xu Y, Cui F*. 2016. Phylogenetic analysis of the ATP-binding cassette transporter family in three mosquito species. Pesticide Biochemistry and Physiology. 132: 118-124.
  38. Lu H, Yang P, Xu Y, Luo L, Zhu J, Cui N, Kang L*, Cui F*. 2016. Performances of survival, feeding behavior, and gene expression in aphids reveal their different fitness to host alteration. Scientific Reports. 6:19344.
  39. Zhang F, Li Q, Chen X, Huo Y, Guo H, Song Z, Cui F, Zhang L, Fang R. 2016. Roles of the Laodelphax striatellus Down syndrome cell adhesion molecule in Rice stripe virus infection of its insect vector. Insect Molecular Biology, 25: 413-421.
  40. Pan Y, Zhu J, Luo L, Kang L*, Cui F*. 2015. High expression of a unique aphid protein in the salivary glands of Acyrthosiphon pisum. Physiological and Molecular Plant Pathology. 92: 175-180.
  41. Wang W, Dai H, Zhang Y, Chandrasekar R, Luo L, Hiromasa Y, Sheng C, Peng G, Chen S, Tomich JM, Reese J, Edwards O, Kang L, Reeck G*, Cui F*. 2015. Armet is an effector protein mediating aphid-plant interactions. FASEB Journal. 29: 2032-2045.
  42. Wang W, Luo L, Lu H, Chen S, Kang L, Cui F*. 2015. Angiotensin-converting enzymes modulate aphid–plant interactions. Scientific Reports, 5: 8885.
  43. Cui F#*, Li M#, Chang H, Mao Y, Zhang H, Lu L, Yan S, Lang M, Liu L*, Qiao C. 2015. Carboxylesterase-mediated insecticide resistance: quantitative increase induces broader metabolic resistance than qualitative change. Pesticide Biochemistry and Physiology, 121: 88-96.
  44. Wang W, Liu S, Liu Y, Qiao C, Chen S*, Cui F*. 2015. Over-transcription of genes in a parathion-resistant strain of mosquito Culex pipiens quinquefasciatus. Insect Science, 22: 150-156.
  45. Cui F#, Wang H#, Zhang H, Kang L. 2014. Anoxic stress and rapid cold hardening enhance cold tolerance of the migratory locust. Cryobiology, 69: 243-248.
  46. Guo K, Wang W, Luo L, Chen J, Guo Y, Cui F*. 2014. Characterization of an aphid-specific, cysteine-rich protein enriched in salivary glands. Biophysical Chemistry, 189: 25-32.
  47. Wang X, Fang X, Yang P, Jiang X, Jiang F, Zhao D, Li B, Cui F, Wei J, Ma C, Wang Y, He J, Luo Y, Wang Z, Guo X, Guo W, Wang X, Zhang Y, Yang M, Hao S, Chen B, Ma Z, Yu D, Xiong Z, Zhu Y, Fan D, Han L, Wang B, Chen Y, Wang J, Yang L, Zhao W, Feng Y, Chen G, Lian J, Li Q, Huang Z, Yao X, Lv N, Zhang G, Li Y, Wang J, Wang J, Zhu B, Kang L. 2014. The locust genome provides insight into swarm formation and long-distance flight. Nature Communications, 5: 2957.
  48. Mao Y, Tan F, Yan S, Wu G, Qiao C, Zhang W*, Cui F*. 2013. High-throughput genotyping of single-nucleotide polymorphisms in ace-1 gene of mosquitoes using MALDI-TOF mass spectrometry. Insect Science, 20: 167-174.
  49. Wang Y, Yang P, Cui F, Kang L. 2013. Altered immunity in crowded locust reduced fungal (Metarhizium anisopliae) pathogenesis. PLoS Pathogens, 9: e1003102.
  50. Yan S, Nan P, Cui F, Wu Z, Qiao C. (2013). Distribution and dynamics of esterase alleles in Culex pipiens complex in China. Journal of Asia-Pacific Entomology, 16, 43-48.
  51. Yan L, Yang P, Jiang F, Cui N, Ma E, Qiao C, Cui F*. 2012. Transcriptomic and phylogenetic analysis of Culex pipiens quinquefasciatus for three detoxification gene families. BMC genomics, 13: 609.
  52. Zhang H, Meng F, Qiao C, Cui F*. 2012. Identification of resistant carboxylesterase alleles in Culex pipiens complex via PCR-RFLP. Parasites & Vectors, 5: 209.
  53. Wang H#, Ma Z#, Cui F#, Wang X, Guo W, Lin Z, Yang P, Kang L. 2012. Parental phase status affects the cold hardiness of progeny eggs in locusts. Functional Ecology, 26, 379-389.
  54. Cui F, Smith CM, Reese J, Edwards O, Reeck G. 2012. Polymorphisms in salivary-gland transcripts of Russian wheat aphid biotypes 1 and 2. Insect Science, 19, 429-440.
  55. Gong L#, Cui F#, Sheng C, Lin Z, Reeck G, Xu J, Kang L. 2012. Polymorphism and methylation of four genes expressed in salivary glands of Russian wheat aphid (Homoptera: Aphididae). Journal of Economic Entomology, 105: 232-241.
  56. Lan W, Chen G, Cui F, Tan F, Liu R, Yushupujiang M. 2012. Development of a novel optical biosensor for detection of organophoshorus pesticides based on methyl parathion hydrolase immobilized by metal-chelate affinity. Sensors, 12, 8477-8490.
  57. Yang J, Liu R, Song W, Yang Y, Cui F, Qiao C. 2012. Construction of a genetically engineered microorganism that simultaneously degrades organochlorine and organophosphate pesticides. Applied Biochemistry and Biotechnology, 166: 590-598.
  58. Liu Y, Zhang H, Qiao C, Lu X*, Cui F*. 2011. Correlation between carboxylesterase alleles and insecticide resistance in Culex pipiens complex from China. Parasites & Vectors, 4: 236.
  59. Cui F#, Lin Z#, Wang H, Liu S, Chang H, Reeck G, Qiao C, Raymond M, Kang L. 2011. Two single mutations commonly cause qualitative change of nonspecific carboxylesterases in insects. Insect Biochemistry and Molecular Biology, 41: 1-8.
  60. James C Carolan, Doina Caragea, Karen T Reardon, Navdeep S Mutti, Neal Dittmer, Kirk Pappan, Feng Cui, Marisol Castaneto, Julie Poulain, Carole Dossat, Denis Tagu, John C. Reese, Gerald R. Reeck, Thomas L Wilkinson, and Owain R. Edwards. 2011. Predicted effector molecules in the salivary secretome of the pea aphid (Acyrthosiphon pisum)-A dual transcriptomic-proteomic approach. Journal of Proteome Research, 10: 1505-1518.
  61. Yan S, Cui F, Qiao C. 2009. Structure, function and applications of insect carboxylesterases evolved for insecticide resistance. Protein and Peptide Letters, 16: 1181-1188.
  62. Yan S, Wu Z, Cui F, Zhao Q, Qiao C. 2008. Dynamics of esterase alleles in Culex pipiens complex mosquitoes in Beijing. Journal of Economic Entomology, 101: 1897-1902.
  63. Cui F, Qu H, Cong J, Liu X, Qiao C. 2007. Do mosquitoes acquire organophosphate resistance by functional changes in carboxylesterases? The FASEB Journal, 21: 3584-3591.
  64. Cui F, Weill M, Berthomieu A, Raymond M, Qiao C. 2007. Characterization of novel esterases in insecticide-resistant mosquitoes. Insect Biochemistry and Molecular Biology, 37: 1131-1137.
  65. Cui F, Tan Y, Qiao C. 2007. Filariasis vector in China: insecticide resistance and population structure of mosquito Culex pipiens complex. Pest Management Science, 63: 453-458.
  66. Cui F, Qiao C, Shen B, Marquine M, Weill M, Raymond M. 2007. Genetic differentiation of Culex pipiens in China. Bulletin of Entomological Research, 97: 291-297.
  67. Alout H, Berthomieu A, Cui F, Tan Y, Berticat C, Qiao C, Weill M. 2007. Different amino-Acid substitutions confer insecticide resistance through acetylcholinesterase 1 insensitivity in Culex vishnui and Culex tritaeniorhynchus (Diptera: Culicidae) from China. Journal of Medical Entomology, 44: 463-469.
  68. Cui F, Raymond M, Berthomieu A, Alout H, Weill M, Qiao C. 2006. Recent emergence of insensitive acetylcholinesterase in Chinese populations of the mosquito Culex pipiens. Journal of Medical Entomology, 43: 878-883.
  69. Cui F, Raymond M, Qiao C. 2006. Insecticide resistance of vector mosquitoes in China. Pest Management Science, 62: 1013-1022.
  70. Cui F, Lin L, Qiao C, Xu Y, Marquine M, Weill M, Raymond M. 2006. Insecticide resistance in Chinese populations of the Culex pipiens complex through esterase overproduction. Entomologia Experimentalis et Applicata, 120: 211-220.