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Name:
 ZHAO Jianguo
Subject:
Tel/Fax:
 +86-10-64806259  / 
E-mail:
 zhaojg@ioz.ac.cn, zhaojervis@hotmail.com
Address:
 The State Key Laboratory of Stem cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, P.R.China
More:
 Group of Genetic modification in large animals      
Resume:

Dr. Zhao received his Ph.D. in Animal genetics and breeding (2003) from Northeast Agricultural University and attended Institute of Medical Genetics at Shanghai Jiaotong University as an assistant researcher from 2003 to 2005. He had postdoctoral training in University of New Orleans of US with Dr. Barry Bavister (2005-2007). After that he worked in University of Missouri-Columbia/ National Swine Research and Resource Center as a Research Assistant Professor from 2007 to 2010. He joined the State Key Laboratory of Reproductive Biology of the Institute of Zoology, CAS, as a principle investigator.

Research Interests:

Dr Zhao’s laboratory is committed to building an efficient platform for pig genetic modifications and functional genomics analysis, providing ideal disease models for biomedical research and preclinical transformation, and providing new solutions for pig economic trait improvement and breeding. The main research directions are as follows: 1. Establishment of mutant lines and screening of functional genes by whole-genome random mutagenesis; 2. Genetic modifications by using various genome editing tools to achieve the agricultural and biomedical applications of pigs; 3. Reprogramming mechanism in somatic cell nuclear transfer and epigenetic regulations in the development of porcine early stage embryos.

Awards and Honors:

Professional Activities:

Research Grants:

Selected Publications:
  1. Yang YJ , Zhang Y , Liu Q, Tian SJ , Feng WY, Deng FW, Qin GS , Wang YF * , Zhao JG* .OIVC medium enhances developmental competence in porcine preimplantation embryos. Journal of Integrative Agriculture.2025
  2. Wang X , Liu TX , Zhang Y, Xu LW ,Yuan SL ,Cui AL , Guo WW , Wang YF ,  Yang SM*,Zhao JG*.Genetically modified pigs: Emerging animal models for hereditary hearing loss.Zoological Research.2024, 45(2): 284−291
  3. Wang Z , Wang Y, Zhang Y, Qin GS , Sun WB , Wang AP, Wang YF * , Zhang GP*, Zhao JG*.On-site detection and differentiation of African swine fever virus variants using an orthogonal CRISPR-Cas12b/Cas13a-based assay.iScience.2024,27
  4. Wang XY, Wang Y, Wang Y, Guo YF, Zong RJ, Hu ST, Yue JW, Yao J, Han CS *, Guo JT*,Zhao JG*. Single-cell transcriptomic and cross-species comparison analyses reveal distinct molecular changes of porcine testes during puberty.Communications Biology. 2024,7 (1), 1478
  5. Qi YN, Zhang Y, Tian SJ, Zong RJ, Yan XH, Wang Yu, Wang YF *, Zhao JG*. An optimized prime editing system for efficient modification of the pig genome. SCIENCE CHINA Life Sciences. 2023, (66) 2851-2861
  6. Ma YJ, Wu BB, Chen YH, Ma S, Wang LY, Han TT, Lin XL, Yang FL, Liu C, Zhao JG*, Li W*. CCDC146 is required for sperm flagellum biogenesis and male fertility in mice. Cellular and Molecular Life Sciences. 2023 Dec 1;81(1):1
  7. Dong FC , Wang XG, Huang T , Yin YY, Xiao S, Ma YJ, Wei HF, Wu BB, Zhang RD, Wang LY, Guo XJ, Gao F, Liu C, Liu HB,Zhao JG*,Li W *.CCDC178-mediated cytoskeleton assembly is required for spermiogenesis in mice. Genes & Disease. 2023.09
  8. Qiao CM, He MZ, Wang SM, Jiang XJ , Wang F , Li XJ, Tan S, Chao Z, Xin WS , Gao S , Yuan JL, Li Q, Xu ZC , Zheng XL, Zhao JG*, Liu GL *.Multi-omics analysis reveals substantial linkages between the oral-gut microbiomes and inflamm-aging molecules in elderly pigs. Frontiers in Microbiology. 2023:14
  9. Zhao Y, Chen CH ,Pan JL, Lam Sin Man , Shui GH, Yang SL, Wu TW , Yang N, Tao C, Zhao JG*; Wang, YF*. Adipocyte Rnf20 ablation increases the fast-twitch fibers of skeletal muscle via lysophosphatidylcholine 16:0. Cellular and Molecular Life Science.2023:9
  10. Song R, Wang Y, Zheng Q, Yao J, Cao C, Wang Y*, Zhao JG*. One-step base editing in multiple genes by direct embryo injection for pig trait improvement. Sci China Life Sci. 2022 Ap;65(4):739-752.
  11. Gu P, Hui X, Zheng Q, Gao Y, Jin L, Jiang W, Zhou C, Liu T, Huang Y, Liu Q, Nie T, Wang Y, Wang Y, Zhao JG*, Xu A*. Mitochondrial uncoupling protein 1 antagonizes atherosclerosis by blocking NLRP3 inflammasome-dependent interleukin-1β production. Sci Adv. 2021; 7(50): eabl4024.
  12. Song R, Liu K, Wang Y, Qin G, Xiao M, Liu Y, Yang J, Zong R, Yan X, Zheng Q, Zhang H, Hou N, Yao J, Wang X, Wang Y, Zhao JG*, Zhang X*. A NCSTN heterozygous point mutation pig model simulates familial acne inversa and reveals dysregulated cholesterol biosynthesis via the notch-pAMPK-HMGCR pathway. Science Bulletin.2021, 66(23):2343-2346.
  13. Liang X, Tao C, Pan J, Zhang L, Liu L, Zhao Y, Fan Y, Cao C, Liu J, Zhang J, Lam SM, Shui G, Jin W, Li W, Zhao JG*, Li K*, Wang Y*. Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis. Protein Cell. 2021, 12(6):475-492.
  14. Liu L,Wang Y,Liang X,Wu X,Liu J,Yang S,Tao C,Zhang J,Tian J, Zhao JG*, Wang Y*. Stearoyl-CoA desaturase is essential for porcine adipocyte differentiation. Int J Mol Sci. 2020 Apr 1;21(7): E2446.
  15. Zhao JG, Lai L, Ji W, Zhou Q*. Genome editing in large animals: Current status and future prospects. Natl Sci Rev.2019, 6(3) : 402–420
  16. Hai T, Cao C, Shang H, Guo W, Mu Y, Yang S, Zhang Y, Zheng Q, Zhang T, Wang X, Liu Y, Kong Q, Li K, Wang D, Qi M, Hong Q, Zhang R, Wang X, Jia Q, Wang X, Qin G, Li Y, Luo A, Jin W, Yao J, Huang J, Zhang H, Li M, Xie X, Zheng X, Guo K, Wang Q, Zhang S, Li L, Xie F, Zhang Y, Weng X, Yin Z, Hu K, Cong Y, Zheng P, Zou H, Xin L, Xia J, Ruan J, Li H, Zhao W, Yuan J, Liu Z, Gu W, Li M, Wang Y, Wang H*, Yang S*, Liu Z*, Wei H*, Zhao JG*, Zhou Q*, Meng A*. Pilot study of large-scale production of mutant pigs by ENU mutagenesis. Elife. 2017, 6. pii: e26248.
  17. Lin J, Cao C, Tao C, Ye R, Dong M, Zheng Q, Wang C, Jiang X, Qin G, Yan C, Li K, Speakman JR, Wang Y*, Jin W*, Zhao JG*. Cold adaptation in pigs depends on UCP3 in beige adipocytes. J Mol Cell Biol. 2017 May 9.
  18. Zheng Q, Lin J, Huang J, Zhang H, Zhang R, Zhang X, Cao C, Hambly C, Qin G, Yao J, Song R, Jia Q, Wang X, Li Y, Zhang N, Piao Z, Ye R, Speakman JR, Wang H, Zhou Q, Wang Y, Jin W, Zhao JG*. Reconstitution of UCP1 using CRISPR/Cas9 in the white adipose tissue of pigs decrease fat deposition and improve thermogenic capacity. Proc Natl Acad Sci U S A. 2017;
  19. Zhang Y, Xue Y, Cao C, Huang J, Hong Q, Hai T, Jia Q, Wang X, Qin G, Yao J, Wang X, Zheng Q, Zhang R, Li Y, Luo A, Zhang N, Shi G, Wang Y, Ying H, Liu Z, Wang H, Meng A, Zhou Q, Wei H*, Liu F*, Zhao JG*. Thyroid hormone regulates hematopoiesis via the TR-KLF9 axis. Blood. 2017; 130(20):2161-2170.