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Name:
 WANG Haoyi
Subject:
 Regenerative Medicine
Tel/Fax:
 86-010-82619213 (Beijing)  / 
E-mail:
 wanghaoyi@ioz.ac.cn
Address:
 State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
More:
 Group of Genome Engineering Technology      
Resume:

Haoyi Wang, Principal Investigator, Genetic Engineering Technology Research Group, Institute of Zoology, Chinese Academy of Sciences. He received his PhD from Washington University in St. Louis in 2009 and conducted postdoctoral research at the Whitehead Institute of Massachusetts Institute of Technology from 2009 to 2014. In 2014, he joined the Institute of Zoology, Chinese Academy of Sciences, and set up his own lab. Recently, he has developed multiple novel TnpB gene editing tools and DNA transposon tools, and identified several new families of tandem repeat proteins that bind to DNA. He has established efficient gene editing methods in human pluripotent stem cells and CAR-T cells, and collaborated on clinical studies of gene edited CAR-T cell therapy for solid tumors; Established culture conditions for human naive embryonic stem cells, among other achievements. The relevant research results have been published in journals such as Cell, Nature Biotechnology, Cancer Cell, Cell Stem Cell, Genome Research, and Cell Research. More than 80 SCI academic papers have been published, with total citations exceeding 17000 (according to Google Scholar). In recent years, he has undertaken the National Key Research and Development Program of China as chief scientist, two sub projects of the CAS Strategic Priority Research Program, and the National Natural Science Foundation including China Distinguished Young Scholars Fund, Excellent Young Scholars Fund, General Program, and Ministry of Agriculture and Rural Affairs of China.

EDUCATION

Washington University School of Medicine, St. Louis, MO

Ph.D. in Molecular Cell Biology, received August 2009

Miami University, Oxford, OH

M.S. in Zoology, received May 2003

Xiamen University, Fujian, China

B.S. in Biology, received June 2001

RESEARCH EXPERIENCE

2014-present

Principle Investigator, Institute of Zoology, Chinese Academy of Sciences

Development of novel technologies to achieve more efficient and specific genome engineering.    

2014-2016 

Distinguished Visiting Professor, the Jackson Laboratory, Bar Harbor

Improving gene editing technology for generating mouse models

2009-2013

Postdoctoral Associate with Rudolf Jaenisch, Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

Development and application of site-specific nucleases on genome engineering of hES, hiPS cells and mouse.     

2003-2009

Doctoral Candidate, with Mark Johnston and Rob Mitra, Washington University in St. Louis.

Development and application of transposon “calling cards”, a novel method for determining the genome-wide binding locations of transcription factors. 

2001-2003

Masters Candidate, Laboratory of Dr. Susan Hoffman, Miami University.

Detailed analysis of the organization and evolution of the Cyp2 gene family cluster in mouse. 

Research Interests:

1. Developing novel tools for genome engineering

2. Development of new cell therapy techniques using gene editing technology

Awards and Honors:

Professional Activities:

Research Grants:

Stem Cell Pioneering Research Program of CAS

No. XDA01010409 (2014-2015)

National Natural Science Foundation Project-General Project

No. 31471215 (2015-2018)

High-tech Research and Development Program of China "863" project

No. 2015AA020307 (2015-2017)

Stem Cell Pioneering Research Program of CAS

No. XDA16010503(2017-2022)

National Natural Science Foundation Project-General Project

No. 81773269 (2018-2021)

National Natural Science Foundation for Excellent Young Scholars

No. 31722036(2018-2020)

National Key Research and Development Program of China

No. 2018YFA0107703(2018-2022)

National Key Research and Development Program of China

No. 2019YFA0110000(2019-2023)

From 0 to 1 innovation project of basic frontier science research program of CAS

No. ZDBS-LY- SM005(2019-2023)

Biological Breeding-Major Projects

No. 2023ZD0407401(2023-2025)

Sciences Key Core Technology Breakthrough Pilot Project of CAS

No. XDC0200201(2024-2027)

National Science Fund for Distinguished Young Scholars

No. 32425035 (2025-2029)

Selected Publications:
  1. Tang N*#, Cheng L*, Hao J, Xu B, Pan X, Wei X, Wu H#, Wang H#, “Development of CAR‑T cell therapy for NF1/SWN‑related nerve sheath tumor treatment.” Acta Neuropathologica Communications, 2025; 13(1):45.
  2. Zhang X*, Zhang C*, Lu S*, Dong J*, Tang N, Wang Y, Han W, Pan X, Zhang X, Liu D, Shyh-Chang N, Wang Y, Feng G, Wang H, “Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state.” Cell reports Medicine, 2024;5:101869
  3. Hu X*, Zhang X*, Sun W*#, Liu C*, Deng P, Cao Y, Zhang C, Xu N, Zhang T, Zhang Y, Liu J, Wang H#，“Systematic discovery of DNA-binding tandem repeat proteins”. Nucleic Acids Research, 2024; 52(17), 10464–10489.
  4. Zhang X*, Hu X*, Zhang T, Yang L, Liu C, Xu N, WangH#, Sun W#, “PLM_Sol: predicting protein solubility by benchmarking multiple protein language models with the updated Escherichia coli protein solubility dataset.” Briefings in Bioinformatics, 2024; 25(5), bbae404.
  5. Zhang T*, Tan S*, Tang N*, Li Y* , Zhang C , Sun J , Guo Y, Gao H, Cai Y, Sun W, Wang C, Fu L, Ma H , Wu Y, Hu X, Zhang X, Gee P , Yan W , Zhao Y, Chen Q , Guo B , Wang H#, Zhang Y#，“Heterologous survey of 130 DNA transposons in human cells highlights their functional divergence and expands the genome engineering toolbox.” Cell, 2024; 187(14):3741-3760. e30.
  6. Zhang X, Wang H, “Unlocking CAR T cell potential: Inosine-induced stemness and enhanced potency.” Cancer Cell, 2024; 42(2): 175-177.
  7. Lin Q, Cao Y, Han G, Sun W, Weng W, Chen H#, Wang H#, Kong J#, “Programmable Clostridium perfringens Argonaute-Based, One-Pot Assay for the Multiplex Detection of miRNAs.” Analytical Chemistry, 2023; 95 (36): 13401-13406.
  8. Xiang G#*, Li Y*, Sun J*, Huo Y*, Cao S, Cao Y, Guo Y, Yang L, Cai Y, Zhang Y#, Wang H#,“Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature genome editors.” Nature Biotechnology, 2024; 42（5）: 745–757.
  9. Deng P*, Tan S*, Yang Q, Fu L, Wu Y, Zhu H, Sun L, Bao Z, Lin Y, Zhang Q, Wang H, Wang J#, Liu J#,“Structural RNA components supervise the sequential DNA cleavage in R2 retrotransposon. ” Cell , 2023 Jun; 186 (13): 2865-2879. e20.
  10. Wang Y*, Yang N*, Sun W*, Zhao C, Hu X, Lu S, Cao S, Wang N, Hai T, Feng G#, An C#, Wang H#, “Characterisation of X chromosome status of human extended pluripotent stem cells.” Cell proliferation. 2023 Mar; Online.
  11. Song Y*, Chen M*, Zhang Y, Li J, Liu B, Li N, Qiao M, Wang N, Cao Y, Lu S, Chen J, Sun W#, Gao F#, Wang H#. “Loss of circSRY reduces γH2AX level in germ cells and impairs mouse spermatogenesis.” Life Science Alliance, 2022 Nov; ;6(2): e202201617. doi: 10.26508/lsa. 202201617. 
  12. Zhang X*, Zhang C*, Qiao M*, Cheng C, Tang N, Lu S, Sun W, Xu B, Cao Y, Wei X, Wang Y, Han W, Wang H. “Depletion of BATF in CAR-T cells enhances antitumor activity by inducing resistance against exhaustion and formation of central memory cells.” Cancer Cell, 2022; 40(11):1407-1422.e7.
  13. Li G*, Li X*, Zhuang S*, Wang L*, Zhu Y*, Chen Y*, Sun W*, Wu Z*, Zhou Z*, Chen J#, Huang X#, Wang J#, Li D#, Li W#, Wang H#, Wei W#. “Gene editing and its applications in biomedicine.” Science China-Life Sciences, 2022 Apr; 65(4):660-700.
  14. Wu T*, Cao Y*, Liu Q, Wu X, Shang Y, Piao J, Li Y, Dong Y, Liu D, Wang H #, Liu J#, Ding B#. “Genetically Encoded Double-Stranded DNA-Based Nanostructure Folded by a Covalently Bivalent CRISPR/dCas System.” JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2022 Apr; 144(14):6575-6582.
  15. Guo Y*, Xu B*, Wu Z*, Bo J#, Tong C, Chen D, Wang J, Wang H#, Wang Y,# Han W#. “Mutant B2M-HLA-E and B2M-HLA-G fusion proteins protects universal chimeric antigen receptor-modified T cells from allogeneic NK cell-mediated lysis.” EUROPEAN JOURNAL OF IMMUNOLOGY, 2021; 51(10):2513-2521.
  16. Wang Z*, Li N*, Feng K*, Chen M, Zhang Y, Liu Y, Yang Q, Nie J, Tang N, Zhang X, Cheng C, Shen L, He J, Ye X, Cao W#, Wang H#, Han W#. “Phase I study of CAR-T cells with PD-1 and TCR disruption in mesothelin-positive solid tumors.” Cellular & Molecular Immunology, 2021 Aug; 18(9): 2188-2198.
  17. Li N, Tang N, Cheng C, Hu T, Wei X, Han W, Wang H. “Improving the anti-solid tumor efficacy of CAR-T cells by inhibiting adenosine signaling pathway.” OncoImmunology, 2020 Sep 24 ; 9(1): e1824643.
  18. Zhang X*, Cheng C*, Sun W, Wang H. “Engineering T Cells Using CRISPR/Cas9 for Cancer Therapy.” Methods Mol Biol, 2020; 2115: 419-433. 
  19. Sun W, Wang H. (2020). “Recent advances of genome editing and related technologies in China”. Gene Therapy, 2020 Aug; 27(7-8): 312-320. 
  20. An C*, Feng G*, Zhang J, Cao S, Wang Y, Wang N, Lu F, Zhou Q, Wang H. “Overcoming Autocrine FGF Signaling-Induced Heterogeneity in Naive Human ESCs Enables Modeling of Random X Chromosome Inactivation” Cell Stem Cell, 2020 Sep;27(3):482–497.
  21. Tang N*, Cheng C*, Zhang X, Qiao M, Li N, Mu W, Wei X, Han W, Wang H. “TGFβ Inhibition via CRISPR Promotes the Long-Term Efficacy of CAR-T Cells Against Solid Tumors” JCI Insight, 2020 Feb;5(4): e133977. 
  22. Cao Y*, Sun W*, Wang J, Sheng G, Xiang G, Zhang T, Shi W, Li C, Wang Y, Zhao F#, Wang H#. “Argonaute proteins from human gastrointestinal bacteria catalyze DNA-guided cleavage of single- and double- stranded DNA at 37 °C.” Cell Discovery, 2019 Jul 30;5:38.
  23. Wang H#，Yang H#. “Gene-edited babies: What went wrong and what could go wrong.” PLOS Biology, 2019 Apr 30;17(4):e3000224.
  24. Mu W*, Tang N*, Cheng C*, Sun W, Wei X, Wang H#. “In vitro transcribed sgRNA causes cell death by inducing interferon release.” Protein & Cell,2019 Jun;10(6):461-465.
  25. Cheng C*, Tang N*, Li J, Cao S, Zhang T, Wei X, Wang H#. “ Bacteria-free minicircle DNA system to generate integration-free CAR-T cells.” Journal of Medical Genetics, 2019 Jan;56(1):10-17. 
  26. Xiang G*, Ren J*, Hai T*, Fu R, Yu D, Wang J, Li W, Wang H#, Zhou Q#. “Editing porcine IGF2 regulatory element improved meat production in Chinese Bama pigs.” Cellular and Molecular Life Sciences. 2018 Dec;75(24):4619-4628.  
  27. Xiang G, Wang H#. “Extended pluripotent stem cells facilitate mouse model generation.” Protein & Cell. 2018 Aug 21.doi: 10.1007/s13238-018-0573-0.
  28. Mu W, Zhang Y, Xue X, Liu L, Wei X, Wang H#.” 5′ capped and 3′ polyA-tailed sgRNAs enhance the efficiency of CRISPR-Cas9 system.” Protein & Cell, 2018.Jun;10(3):223–228.
  29. Zhang Y, Mu W, Wang H#. “Gene editing in T cell therapy.” Journal of Genetics and Genomics,  2017 Sep;44 (9) :415-422.
  30. Wang H#. “Editing Base in Mouse Model.” Protein & Cell, 2017 Aug;8(8):558-559.
  31. Wang W, Zhang Y, Wang H#. “Generating Mouse Models Using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput.” Methods in Molecular Biology, 2017;1605:219-230. 
  32. Zhang Y, Zhang X, Cheng C, Mu W, Liu X, Li N, Wei X, Liu X, Xia C, Wang H#. “CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells.” Frontiers of Medicine, 2017 Dec;11(4):554-562.
  33. Xiang G, Zhang X, An C, Cheng C, Wang H#. “Temperature effect on CRISPR-Cas9 mediated genome editing.” Journal of Genetics and Genomics, 2017 Apr ;44(4):199-205.
  34. Liu X, Zhang Y, Cheng C, Cheng WA, Zhang X, Li N, Xia C, Wei X, Liu X, Wang H#. “CRISPR-Cas9 mediated multiplex gene editing in CAR-T cells.” Cell Research, 2017 Jan;27(1):154-157.
  35. Burgess S, Cheng L#, Gu F, Huang J, Huang Z, Lin S#, Li J, Li W, Qin W, Sun W, Songyang Z, Wei W#, Wu Q, Wang H#, Wang X, Xiong JW, Xi J, Yang H, Zhou B, Zhang B. “Questions about NgAgo” Protein Cell, 2016 Dec;7(12):913-915.
  36. Wang W,Kutny PM, Byers SL, Longstaff CJ, DaCosta MJ, Pang C, Zhang Y, Taft RA, Buaas FW, Wang H#. “Delivery of Cas9 protein into mouse zygotes through a series of electroporation dramatically increased the efficiency of model creation.” Journal of Genetics and Genomics, 2016 May 20;43(5):319-27.
  37. Qin W, Kutny PM, Maser RS, Dion SL, Lamont JD, Zhang Y, Perry GA, Wang H#.“Generating Mouse Models Using CRISPR-Cas9 Mediated Genome Editing.”Current Protocols in Mouse Biology, 2016 Mar 1;6(1):39-66. 
  38. Cheng AW*#, Jillette N*, Lee P, Plaskon D, Fujiwara Y, Wang W, Taghbalout A, Wang H#. “Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling.” Cell Research, 2016 Feb;26(2):254-7. 
  39. Wiles MV, Qin W, Cheng AW, Wang H#. “CRISPR-Cas9 mediated genome editing and guide RNA design.” Mammalian Genome, 2015 Oct;26(9-10):501-10.
  40. Qin W, Dion SL, Kutny PM, Zhang Y, Cheng AW, Jillette NL, Malhotra A, Geurts AM, Chen YG, Wang H#. “Efficient CRISPR/Cas9-mediated genome editing in mice by zygote electroporation of nuclease.” Genetics, 2015 Jun;200(2):423-30.
  41. Theunissen T*, Powell B*, Wang H*, Mitalipova M, Faddah D, Reddy J, Fan Z, Maetzel D, Ganz K, Shi L, Lungjangwa T, Imsoonthornruksa S, Stelzer Y, Rangarajan S, D’Alessio A, Zhang J, Gao Q, Dawlaty M, Young R, Gray N, Jaenisch R. “Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency.”Cell Stem Cell, 2014 Oct 2; 15(4):471-87.
  42. Yang H, Wang H, Jaenisch R. “Generating genetically modified mice using CRISPR/Cas-mediated genome engineering.” Nature protocols, 2014 Aug; 9(8):1956-68.
  43. Maetzel D*,Sarkar S*, Wang H*, Mosleh LA, Cheng AW, Xu P, Gao Q, Mitalipova M, Jaenisch R. “Genetic and chemical correction of cholesterol accumulation and impaired autophagy in hepatic and neural cells derived from Niemann-Pick Type C patient-specific iPS cells.” Stem Cell Reports, 2014 May 15; 2(6):866-80.
  44. Cheng AW*, Wang H*, Yang H, Shi L, Katz Y, Rangarajan S, Theunissen TW, Shivalila CS, Dadon DB, Jaenisch R. “Multiplexed activation of endogenous genes by CRISPR-on, a RNA-guided transcriptional activator system.” Cell Research, 2013 October 1; 23(10): 1163-1171.
  45. Yang H*, Wang H*, Shivalila CS*, Cheng AW, Shi L, Jaenisch R. “One-step generation of mice carrying reporter and conditional allele by CRISPR/Cas mediated genome editing” Cell, 2013 September 12; 154(6):1370-1379.
  46. Faddah D, Wang H, Buganim Y, Cheng AW, Jaenisch R. “Expression of Nanog is biallelic and equally variable as other pluripotency factors.” Cell Stem Cell, 2013 Jul 3;13(1):23-9.
  47. Wang H*, Hu YC*, Markoulaki S, Welstead GG, Shivalila CS, Cheng AW, Pyntikova T, Dadon D, Voytas DF, Bogdanove AJ, Page DC, Jaenisch R. “TALEN-mediated editing of the Mouse Y Chromosome.” Nature Biotechnology, 2013 Jun; 31(6):530-2.
  48. Wang H*, Yang H*, Shivalila CS*, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R. “One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas mediated genome engineering.” Cell, 2013 May 9;153(4):910-8.

 

*These authors contribute equally to this work.