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Name:
 LI Wei
Subject:
 Developmental Biology
Tel/Fax:
 +86-10-64807297  /  +86-10-64807306
E-mail:
 liwei@ioz.ac.cn
Address:
 Institute of Zoology, Chinese Academy of Sciences (CAS).
Institute for Stem Cell and Regenerative Medicine, CAS, Yard A 3, Datun Road, Chaoyang District, Beijing 100101, P. R. China
More:
 Group of Stem Cells and Cell Cycle Regulation      
Resume:

Education:

2006-2012: Ph.D. in Developmental Biology, University of Chinese Academy of Sciences / Institute of Zoology, CAS

2002-2006: B.Sc. in Life Sciences, College of Life Sciences, Wuhan University

Employment:

2013.02-2015.12: Associate professor, Institute of Zoology, CAS

2016.01-Ongoing: Professor, Institute of Zoology, CAS

2017.09-Ongoing: Vice Director of State Key Laboratory of Stem Cell and Reproductive Biology

2024.01-Ongoing: Director of Key Laboratory of Organ Regeneration and Reconstruction, CAS

Research Interests:

The long-term goal of my research is to develop mammalian engineering biology through combining genome editing, stem cells, and embryo manipulation technologies, to explore the mechanisms and approaches of mammalian organ regeneration, and to develop novel biotherapies for unmet medical needs.

Awards and Honors:

2013: Outstanding Science and Technology Achievement Prize of the CAS (Major Contributor)

2013: International Award of the Japanese Association for Laboratory Animal Science

2014: Excellent Young Scientists Fund of the National Natural Science Foundation of China

2015: LU JIAXI Young Talents Award, CAS

2018: Young Scientists Award of the CAS

2019-2023: Young Affiliate of TWAS (The World Academy of Sciences for the advancement of science in developing countries)

2020: Outstanding Science and Technology Achievement Prize of the CAS (Outstanding Contributor)

2022: National Science Fund for Distinguished Young Scholars

2023: Zhu Li Yuehua Excellent Teacher Award, CAS

Professional Activities:

Research Grants:

Selected Publications:

(# denotes first authors; * denotes corresponding authors)

  1. Chen Y#, Luo S#, Hu Y#, Mao B#, Wang X#, Lu Z#, Shan Q, Zhang J, Wang S, Feng G, Wang C, Liang C, Tang N, Niu R, Wang J, Han J, Yang N, Wang H, Zhou Q*, Li W*. All-RNA-mediated targeted gene integration in mammalian cells with rationally engineered R2 retrotransposons. Cell 2024 Aug 22;187(17):4674-4689.e18. doi: 10.1016/j.cell.2024.06.020. Epub 2024 Jul 8.
  2. Cui T#, Cai B#, Tian Y, Liu X, Liang C, Gao Q, Li B, Ding Y, Li R, Zhou Q, Li W*, Teng F*. Therapeutic In Vivo Gene Editing Achieved by a Hypercompact CRISPR-Cas12f1 System Delivered with All-in-One Adeno-Associated Virus. Advanced Science (Weinh) 2024 May;11(19):e2308095. doi: 10.1002/advs.202308095. Epub 2024 Feb 26. (Cover story)
  3. Wang C#, Fang S#, Chen Y#, Tang N, Jiao G, Hu Y, Li J, Shan Q, Wang X, Feng G, Zhou Q*, Li W*. High-efficiency targeted transgene integration via primed micro-homologues. Cell Discovery 2023 Jul 4;9(1):69. doi: 10.1038/s41421-023-00552-0.
  4. Wang LB#, Li ZK#, Wang LY#, Xu K#, Ji TT#, Mao YH, Ma SN, Liu T, Tu CF, Zhao Q, Fan XN, Liu C, Wang LY, Shu YJ, Yang N, Zhou Q*, Li W*. A sustainable mouse karyotype created by programmed chromosome fusion. Science 2022 Aug 26;377(6609):967-975. doi: 10.1126/science.abm1964. Epub 2022 Aug 25.
  5. Chen Y#, Hu Y#, Wang X#, Luo S, Yang N, Chen Y, Li Z, Zhou Q*, Li W*. Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing. Innovation (Camb). 2022 May 26;3(4):100264. doi: 10.1016/j.xinn.2022.100264.
  6. He ZQ#, Li YH#, Feng GH#, Yuan XW#, Lu ZB#, Dai M, Hu YP, Zhang Y, Zhou Q*, Li W*. Pharmacological Perturbation of Mechanical Contractility Enables Robust Transdifferentiation of Human Fibroblasts into Neurons. Advanced Science (Weinh). 2022 May;9(13):e2104682. doi: 10.1002/advs.202104682.
  7. Hu Y#, Chen Y#, Xu J#, Wang X, Luo S, Mao B, Zhou Q*, Li W*. Metagenomic discovery of novel CRISPR-Cas13 systems. Cell Discovery 2022 Oct 11;8(1):107. doi: 10.1038/s41421-022-00464-5.
  8. Guo L, Sun X, Wang X, Liang C, Jiang H, Gao Q, Dai M, Qu B, Fang S, Mao Y, Chen Y, Feng G, Gu Q, Wang RR*, Zhou Q*, Li W*. SARS-CoV-2 detection with CRISPR diagnostics. Cell Discovery 2020 May 19;6:34. doi: 10.1038/s41421-020-0174-y.
  9. Wang LY#, Li ZK#, Wang LB#, Liu C#, Sun XH#, Feng GH#, Wang JQ, Li YF, Qiao LY, Nie H, Jiang LY, Sun H, Xie YL, Ma SN, Wan HF, Lu FL*, Li W*, Zhou Q*. Overcoming Intrinsic H3K27me3 Imprinting Barriers Improves Post-implantation Development after Somatic Cell Nuclear Transfer. Cell Stem Cell 2020 Aug 6;27(2):315-325.e5. doi: 10.1016/j.stem.2020.05.014.
  10. Qu B#, Wu S#, Jiao G#, Zou X#, Li Z#, Guo L#, Sun X, Huang C, Sun Z, Zhang Y, Li H, Zhou Q, Sui R*, Li W*. Treating Bietti crystalline dystrophy in a high-fat diet-exacerbated murine model using gene therapy. Gene Therapy 2020 Aug; 27(7-8):370-382.doi: 10.1038/s41434-020-0159-3.
  11. Mao J#, Xu K#, Han J#, Feng G, Zhang Y*, Li W*. Rapid construction of a whole-genome mutant library by combining haploid stem cells and inducible self-inactivating PiggyBac transposon. Protein & Cell 2020 Jun;11(6):452-457. doi: 10.1007/s13238-020-00702-0.
  12. Teng F#, Guo L#, Cui T, Wang XG, Xu K, Gao Q, Zhou Q*, Li W*. CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity. Genome Biology 2019b Jul 1;20(1):132. doi: 10.1186/s13059-019-1742-z.
  13. Teng F#, Li J#, Cui T#, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou Q*, Li W*. Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds. Genome Biology 2019a Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8.
  14. Li Y#, Wang L#, Zhang L#, He Z#, Feng G, Sun H, Wang J, Li Z, Liu C, Han J, Mao J, Li P, Yuan X, Jiang L, Zhang Y*, Zhou Q*, Li W*. Cyclin B3 is required for metaphase to anaphase transition in oocyte meiosis I. Journal Of Cell Biology 2019 May 6;218(5):1553-1563. doi: 10.1083/jcb.201808088.
  15. Wang J#, Wang L#, Feng G#, Wang Y#, Li Y, Li X, Liu C, Jiao G, Huang C, Shi J, Zhou T, Chen Q, Liu Z, Li W*, Zhou Q*. Asymmetric expression of LincGET biases cell fate in two-cell mouse embryos. Cell 2018 Dec 13;175(7):1887-1901.e18. doi: 10.1016/j.cell.2018.11.039.
  16. Teng F#, Cui T#, Feng G, Guo L, Xu K, Gao Q, Li T, Li J, Zhou Q, Li W*. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discovery 2018 Nov 27;4:63. doi: 10.1038/s41421-018-0069-3.
  17. Zhang W#, Wan H#, Feng G#, Qu J#, Wang J, Jing Y, Ren R, Liu Z, Zhang L, Chen Z, Wang S, Zhao Y, Wang Z, Yuan Y, Zhou Q, Li W*, Liu G*, Hu B*. SIRT6 deficiency results in developmental retardation in cynomolgus monkeys. Nature 2018 Aug;560(7720):661-665. doi: 10.1038/s41586-018-0437-z.
  18. Li ZK#, Wang LY#, Wang LB#, Feng GH#, Yuan XW#, Liu C, Xu K, Li YH, Wan HF, Zhang Y, Li YF, Li X, Li W*, Zhou Q*, Hu BY*. Generation of bimaternal and bipaternal mice from hypomethylated haploid escs with imprinting region deletions. Cell Stem Cell 2018 Nov 1;23(5):665-676.e4. doi: 10.1016/j.stem.2018.09.004.
  19. Xu K#, Yang Y#, Feng GH#, Sun BF#, Chen JQ#, Li YF, Chen YS, Zhang XX, Wang CX, Jiang LY, Liu C, Zhang ZY, Wang XJ, Zhou Q*, Yang YG*, Li W*. Mettl3-mediated m6A regulates spermatogonial differentiation and meiosis initiation. Cell Research 2017 Sep;27(9):1100-1114. doi: 10.1038/cr.2017.100. (Cover story)
  20. Li X#, Cui XL#, Wang JQ#, Wang YK, Li YF, Wang LY, Wan HF, Li TD, Feng GH, Shuai L, Li ZK, Gu Q, Hao J, Wang L, Zhao XY, Liu ZH, Wang XJ, Li W*, Zhou Q*. Generation and application of mouse-rat allodiploid embryonic stem cells. Cell 2016 Jan 14;164(1-2):279-292. doi: 10.1016/j.cell.2015.11.035.
  21. Wan HF#, Feng CJ#, Teng F#, Yang SH#, Hu BY, Niu YY, Xiang P, Fang WZ, Ji WZ, Li W*, Zhao XY*, Zhou Q*. One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system. Cell Research 2015 Feb;25(2):258-261. doi: 10.1038/cr.2014.158.
  22. Li W#, Li X#, Li TD#, Jiang MG#, Wan HF, Luo GZ, Feng CJ, Cui XL, Teng F, Yuan Y, Zhou Q, Gu Q, Shuai L, Sha JH, Xiao YM, Wang L, Liu ZH, Wang XJ, Zhao XY, Zhou Q*. Genetic modification and screening in rat using haploid embryonic stem cells. Cell Stem Cell 2014 Mar 6;14(3):404-414. doi: 10.1016/j.stem.2013.11.016.
  23. Li W#, Teng F#, Li T, Zhou Q*. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nature Biotechnology 2013 Aug;31(8):684-686. doi: 10.1038/nbt.2652.
  24. Li W#, Shuai L#, Wan H#, Dong M#, Wang M, Sang L, Feng C, Luo GZ, Li T, Li X, Wang L, Zheng QY, Sheng C, Wu HJ, Liu Z, Liu L, Wang L, Wang XJ, Zhao XY*, Zhou Q*. Androgenetic haploid embryonic stem cells produce live transgenic mice. Nature 2012 Oct 18;490(7420):407-411. doi: 10.1038/nature11435.
  25. Zhao XY#, Li W#, Lv Z#, Liu L, Tong M, Hai T, Hao J, Guo CL, Ma QW, Wang L, Zeng F*, Zhou Q*. iPS cells produce viable mice through tetraploid complementation. Nature 2009 Sep 3;461(7260):86-90. doi: 10.1038/nature08267.