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Name:
 GUO Jingtao
Subject:
 Developmental Biology
Tel/Fax:
 +86-10-64807310  / 
E-mail:
 jingtao.guo@ioz.ac.cn
Address:
 The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences,1 Beichen West Road, Chaoyang District, Beijing 100101, P.R.China
More:
 Stem Cell and Gonadal Development      
Resume:

2021/12 – Present: Professor and Investigator, State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China

2020/07 – 2021/11: Tenure-track Assistant Professor, University of Utah School of Medicine, Salt Lake City, USA

2018/08 – 2020/07: Postdoctoral researcher, University of Utah School of Medicine, Salt Lake City, USA

2013/08 – 2018/06: Ph.D., Oncological Sciences, University of Utah, Salt Lake City, USA (Advisor: Dr. Bradley Cairns)

2013/08 – 2016/05: Graduate Certificate in Genome Science, University of Utah, Salt Lake City, USA

2009/09 – 2013/07: B.S., Biological Sciences, Peking University, Beijing, China

Research Interests:

The ultimate goal of my lab is to gain a better understanding of male germline stem cell and utilize that knowledge to better diagnose and treat male infertility. In recent years, the Guo lab took advantage of the cutting-edge single-cell genomics profiling approaches and made several impactful discoveries on human germline stem cell and testis development. For instance, we identified a novel type of human spermatogonial stem cells (SSCs), termed State 0. We delineated the molecular mechanisms underlying how State 0 SSCs originate in the fetal testis, and how they undergo differentiation to generate sperm. We also uncovered how somatic differentiation guide the differentiation of germ cells through development. We have published over 20 papers in high-impact journals, including Cell Stem Cell, Nature Genetics, Cell Research, Developmental Cell, American Journal of Human Genetics etc. 

My lab has three major research focuses. First, we utilize a combination of cutting-edge genomics and molecular techniques to understand the normal development and physiology of human germline and testis. Second, we want to develop novel molecular and computational tools to diagnose human male infertility and germ cell tumor more precisely, aiming to offer better treatment for the patients. Last, we are interested to utilize the knowledge learned from our research to establish novel in vivo and in vitro models to better study human SSCs and germline.

Awards and Honors:

Professional Activities:

Research Grants:

Selected Publications:

（#co-first author, *corresponding author）

  1. Cui L#, Nie X#, Guo YX#, Ren P, Guo YF, Wang X, Li R, Hotaling JM, Cairns BR*, Guo J*. Single-cell transcriptomic atlas of human testis across the reproductive lifespan. Nature Aging. 2025 Mar 3. (On Line)
  2. Jiang Q, Cui L, Nie X, Cai H, Zhang W, Lu X, Guo Y, Hotaling JM, Cairns BR, Wang X*, Guo J*. A single-cell transcriptome atlas characterizes the immune landscape of human testes during aging. Aging Cell. 2025 Mar 6;e70032.
  3. Cui L#, Lin S#, Yang X#, Xie X#, Wang X#, He N#, Yang J, Zhang X, Lu X, Yan X, Guo Y, Zhang B, Li R, Miao H, Ji M, Zhang R, Yu L*, Xiao Z*, Wei Y*, Guo J*. Spatial transcriptomic characterization of a Carnegie stage 7 human embryo. Nat Cell Biol. 2025 Feb;27(2):360-369.
  4. Wang X#, Cheng L#, Lu X#, Jin H#, Cui L, Guo Y, Guo J*, Xu EY*. Cross-species comparative single-cell transcriptomics highlights the molecular evolution and genetic basis of male infertility. Cell Rep. 2024 Dec 30;44(1):115118.
  5. Wang X#, Wang Y#, Wang Y#, Guo Y#, Zong R, Hu S, Yue J, Yao J, Han C*, Guo J*, Zhao J*. Single-cell transcriptomic and cross species comparison analyses reveal distinct molecular changes of porcine testes during puberty. Commun Biol. 2024 Nov 9;7(1):1478.
  6. Wang X#, Liu Q#, Zhuang Z#, Cheng J, Zhang W, Jiang Q, Guo Y, Li R, Lu X, Cui L, Weng J, Tang Y, Yue J, Gao S, Hong K, Qiao J, Jiang H*, Guo J*, Zhang Z*. Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling. Cell Rep Med. 2024 Sep 17;5(9):101709.
  7. Cui Y, Harteveld F, Ba Omar HAM, Yang Y, Bjarnason R, Romerius P, Sundin M, Norén Nyström U, Langenskiöld C, Vogt H, Henningsohn L, Frisk P, Vepsäläinen K, Petersen C, Mitchell RT, Guo J, Alves-Lopes JP, Jahnukainen K, Stukenborg JB. Prior exposure to alkylating agents negatively impacts testicular organoid formation in cells obtained from childhood cancer patients. Hum Reprod Open. 2024 Aug 13;2024(3):hoae049.
  8. Xiao Z#, Cui L#, Yuan Y#, He N#, Xie X, Lin S, Yang X, Zhang X, Shi P, Wei Z, Li Y, Wang H, Wang X*, Wei Y*, Guo J*, Yu L*. 3D reconstruction of a gastrulating human embryo. Cell. 2024 May 23;187(11):2855-2874.e19.
  9. Lu X#, Luo Y#, Nie X#, Zhang B, Wang X, Li R, Liu G, Zhou Q, Liu Z, Fan L, Hotaling JM, Zhang Z*, Bo H*, Guo J*. Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment. Nat Commun. 2023 Dec 20;14(1):8462.
  10. Wamaitha SE#, Nie X#, Pandolfi EC, Wang X, Yang Y, Stukenborg JB, Cairns BR, Guo J*, Clark AT*. Single-cell analysis of the developing human ovary defines distinct insights into ovarian somatic and germline progenitors. Dev Cell. 2023 Oct 23;58(20):2097-2111.e3.
  11. Matilionyte G, Forsyth G, Guo J, Rimmer MP, Hermann BP, Anderson RA, Mitchell RT. Granulocyte-colony stimulating factor does not prevent in vitro cisplatin-induced germ cell reduction in immature human and mouse testis.  BMC Cancer. 2023 Mar 16 ;23(1):251.
  12. Nie X, Munyoki SK, Sukhwani M, Schmid N, Missel A, Emery BR, DonorConnect, Stukenborg JB, Mayerhofer A, Orwig KE, Aston KI, Hotaling JM, Cairns BR*, Guo J*. Single-Cell Analysis of Human Testis Aging and Correlation with Elevated Body Mass Index. Dev Cell. 2022 May 9;57(9):1160-1176.e5.
  13. Wang X, Cairns BR*, Guo J*. When spermatogenesis meets human aging and elevated body mass. Life Med. 2022 Jul 9;1(3):267-269.
  14. Mahyari E#, Guo J#*, Lima AC, Lewinsohn DP, Stendahl AM, Vigh-Conrad KA, Nie X, Nagirnaja L, Rockweiler NB, Carrell DT, Hotaling JM, Aston KI, Conrad DF*. Comparative single-cell analysis of biopsies clarifies pathogenic mechanisms in Klinefelter syndrome. Am J Hum Genet. 2021 Oct 7;108(10):1924-1945.
  15. Patel DP, Punjani N, Guo J, Alukal JP, Li PS, Hotaling JM*. The impact of SARS-CoV-2 and COVID-19 on male reproduction and men's health. Fertil Steril. 2021 Apr;115(4):813-823.
  16. Guo J*, Sosa E, Chitiashvili T, Nie X, Rojas EJ, Oliver E; DonorConnect; Plath K, Hotaling JM, Stukenborg JB, Clark AT*, Cairns BR*. Single-cell analysis of the developing human testis reveals somatic niche cell specification and fetal germline stem cell establishment. Cell Stem Cell. 2021 Apr 1;28(4):764-778.e4.
  17. Tharmalingam MD, Matilionyte G, Wallace WHB, Stukenborg JB, Jahnukainen K, Oliver E, Goriely A, Lane S, Guo J, Cairns B, Jorgensen A, Allen CM, Lopes F, Anderson RA, Spears N, Mitchell RT. Cisplatin and carboplatin result in similar gonadotoxicity in immature human testis with implications for fertility preservation in childhood cancer. BMC Med. 2020 Dec 4 ;18(1):374.
  18. Murphy PJ#*, Guo J#, Jenkins TG, James ER, Hoidal JR, Huecksteadt T, Broberg DS, Hotaling JM, Alonso DF, Carrell DT, Cairns BR*, Aston KI*. NRF2 loss recapitulates heritable impacts of paternal cigarette smoke exposure. PLoS Genet. 2020 Jun 10;16(6):e1008756.
  19. Guo J, Nie X, Giebler M, Mlcochova H, Wang Y, Grow EJ; DonorConnect; Kim R, Tharmalingam M, Matilionyte G, Lindskog C, Carrell DT, Mitchell RT, Goriely A, Hotaling JM, Cairns BR*. The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty. Cell Stem Cell. 2020 Feb 6;26(2):262-276.e4.
  20. Guo J*, Cairns BR. Isolation and Enrichment of Spermatogonial Stem Cells From Human Testis Tissues. Curr Protoc Stem Cell Biol. 2019 Jun;49(1):e77.
  21. Guo J, Grow EJ, Mlcochova H, Maher GJ, Lindskog C, Nie X, Guo Y, Takei Y, Yun J, Cai L, Kim R, Carrell DT, Goriely A, Hotaling JM, Cairns BR*. The adult human testis transcriptional cell atlas. Cell Res. 2018 Dec;28(12):1141-1157.
  22. Guo J, Grow EJ, Yi C, Mlcochova H, Maher GJ, Lindskog C, Murphy PJ, Wike CL, Carrell DT, Goriely A, Hotaling JM, Cairns BR*. Chromatin and Single-Cell RNA-Seq Profiling Reveal Dynamic Signaling and Metabolic Transitions during Human Spermatogonial Stem Cell Development. Cell Stem Cell. 2017 Oct 5;21(4):533-546.e6.