Research Interests:

Awards and Honors:

Professional Activities:

Research Grants:

2019~2023, “The molecular mechanisms of the key steps in reproduction”, MOST 2020~2023, “The molecular mechanism of BEND2 in the meiosis of mouse spermatogenic cells “, Natural Science Foundation of China 2018~2021, “Induction of spermatogonial stem cell-like cells from mouse iPS cells by using miRNAs”, Natural Science Foundation of China 2013~2016, “The mechanism of the generation of piRNAs from mRNAs”, Natural Science Foundation of China 2013~2017, “973 Program: The mechanisms of primordial germ cell specification and gonadal development”, MOST 2012~2016, “The mechanisms of in vitro differentiation of multipotent stem cells to germ cells”, MOST

Selected Publications:

Ning Y, Duo S, Lin X, Zhang H, Fei J, Zhang B, Zeng Y, Xie D, Chen J, Liu X, Han C*. Transcription factor PBX4 regulates limb development and haematopoiesis in mice. Cell Prolif. 2024 Jan 17:e13580. doi: 10.1111/cpr.13580. Epub ahead of print. PMID: 38230761. Li J, Lin X, Xie L, Zhao J, Han C*, Deng H*, Xu J*，A CRISPR/Cas9-based kinome screen identifies ErbB signaling as a new regulator of human naive pluripotency and totipotency，Life Medicine, 2023, lnad037, https://doi.org/10.1093/lifemedi/lnad037 Yan L, Chen J, Ning Y, Hac C*. Loss of RBFOX2 inhibits meiotic initiation in male mice[J]. Chinese Journal of Biotechnology, 2023, 39(10): 4108-4122 Lu C, Qin C, Fu Z, Wang L, Yi Y, Xin M, Zhen X, Han C*, Lu C*. Metabolic dysregulation in patients with premature ovarian insufficiency revealed by integrated transcriptomic, methylomic and metabolomic analyses. Clin Transl Med. 2022 Oct;12(10):e1006. Ma, L., Xie, D., Luo, M., Lin, X., Nie, H., Chen, J., Gao, C., Duo, S., and Han, C. (2022) Identification and characterization of BEND2 as a key regulator of meiosis during mouse spermatogenesis. Science Advances, 8(21):eabn1606. Chen, J., Gao, C., Luo, M., Zheng, C., Lin, X., Ning, Y., Ma, L., He, W., Xie, D., Liu, K., Hong, K., Han, C. (2022) MicroRNA-202 safeguards meiotic progression by preventing premature SEPARASE-mediated REC8 cleavage. EMBO Reports, 17;e54298. Chen, J., Gao, C., Lin, X., Ning, Y., He, W., Zheng, C., Zhang, D., Yan, L., Jiang, B., Zhao, Y., Hossen, MA., Han, C. (2021) The microRNA miR-202 prevents precocious spermatogonial differentiation and meiotic initiation during mouse spermatogenesis. Development, 148(24) Zheng, C., Ouyang, YC., Jiang, B., Lin, X., Chen, J., Dong, MZ., Zhuang, X., Yuan, S., Sun, QY., Han C. (2019) Non-canonical RNA polyadenylation polymerase FAM46C is essential for fastening sperm head and flagellum in mice. Biology of Reproduction, 100(6) Zhang, D., Xie, D., Lin, X., Ma, L., Chen, J., Wang, Y., Duo, S., Feng, Y., Zheng, C., Jiang, B., Ning, Y., Han, C.(2018) The transcription factor SOX30 is a key regulator of mouse spermiogenesis. Development,145. doi: 10.1242/dev.164723. Feng, Y., Ning, Y., Lin, X., Zhang, D., Liao, S., Zheng, C., Chen J., Wang Y., Ma L., Xie D., Han C.(2018) Reprogramming p53-Deficient Germline Stem Cells Into Pluripotent State by Nanog. Stem Cells Dev, 27:692-703. Hu, X., Shen, B., Liao, S., Ning, Y., Ma, L., Chen, J., Lin, X., Zhang, D., Li, Z., Zheng, C., Feng, Y., Huang, X., Han C.(2017) Gene knockout of Zmym3 in mice arrests spermatogenesis at meiotic metaphase with defects in spindle assembly checkpoint. Cell Death Di, 8:e2910. Chen, J., Cai, T., Zheng, C., Lin, X., Wang, G., Liao, S., Wang, X., Gan, H., Zhang, D., Hu, X., Wang, S., Li, Z., Feng, Y., Yang, F., and Han, C. (2017) MicroRNA-202 maintains spermatogonial stem cells by inhibiting cell cycle regulators and RNA binding proteins, Nucleic Acids Res. 45(7):4142-4157. Tu, Z., Bayazit, M. B., Liu, H., Zhang, J., Busayavalasa, K., Risal, S., Shao, J., Satyanarayana, A., Coppola, V., Tessarollo, L., Singh, M., Zheng, C., Han, C., Chen, Z., Kaldis, P., Gustafsson, J. A., and Liu, K. (2017) Speedy A-Cdk2 binding mediates initial telomere-nuclear envelope attachment during meiotic prophase I independent of Cdk2 activation, Proc Natl Acad Sci U S A, 114:592-597. Chen, M., Zhang, L., Cui, X., Lin, X., Li, Y., Wang, Y., Wang, Y., Qin, Y., Chen, D., Han, C., Zhou, B., Huff, V., and Gao, F. (2017) Wt1 directs the lineage specification of sertoli and granulosa cells by repressing Sf1 expression, Development 144, 44-53. Wang, S., Wang, X., Ma, L., Lin, X., Zhang, D., Li, Z., Wu, Y., Zheng, C., Feng, X., Liao, S., Feng, Y., Chen, J., Hu, X., Wang, M., and Han, C. (2016) Retinoic Acid Is Sufficient for the In Vitro Induction of Mouse Spermatocytes, Stem cell reports 7, 80-94. Lin, X., Han, M., Cheng, L., Chen, J., Zhang, Z., Shen, T., Wang, M., Wen, B., Ni, T., and Han, C. (2016) Expression dynamics, relationships, and transcriptional regulations of diverse transcripts in mouse spermatogenic cells, RNA biology 13, 1011-1024. Wu, Y., Hu, X., Li, Z., Wang, M., Li, S., Wang, X., Lin, X., Liao, S., Zhang, Z., Feng, X., Wang, S., Cui, X., Wang, Y., Gao, F., Hess, R. A., and Han, C. (2016) Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis, Scientific reports 6, 20435. Yang, Y., Feng, Y., Feng, X., Liao, S., Wang, X., Gan, H., Wang, L., Lin, X., and Han, C. (2016) BMP4 Cooperates with Retinoic Acid to Induce the Expression of Differentiation Markers in Cultured Mouse Spermatogonia, Stem cells international 2016, 9536192. Xue, Y., Lameijer, E. W., Ye, K., Zhang, K., Chang, S., Wang, X., Wu, J., Gao, G., Zhao, F., Li, J., Han, C., Xu, S., Xiao, J., Yang, X., Ying, X., Zhang, X., Chen, W. H., Liu, Y., Zhang, Z., Huang, K., and Yu, J. (2016) Precision Medicine: What Challenges Are We Facing?, Genomics, proteomics & bioinformatics 14, 253-261. Gao, Y., Bai, X., Zhang, D., Han, C., Yuan, J., Liu, W., Cao, X., Chen, Z., Shangguan, F., Zhu, Z., Gao, F., and Qin, Y. (2016) Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis, Nature structural & molecular biology 23, 441-449. #Zhu, L., #Han, C. S., Cao, Z. L., Wang, Z. B., Han, R. G., Wang, B., and Sun, Q. Y. (2015) Confocal Microscopic Analysis of the Spindle and Chromosome Configurations of in vitro-Matured Oocytes from Different Types of Polycystic Ovary Syndrome Patients, Gynecologic and obstetric investigation 80, 179-186. Wang S.，Wang Xx.，Wu Yj.， and *Han, C. (2015) , IGF-1R Signaling Is Essential for the Proliferation of Cultured Mouse Spermatogonial Stem Cells by Promoting the G2/M Progression of the Cell Cycle，Stem Cells and Development 24,471-483 *Han, C. (2014) , Which one is the real matchmaker for the pair?，Asian Journal of Andrology 16,667-668. Li, Y., Wang, X., Feng, X., Liao, S., Zhang, D., Cui, X., Gao, F., and *Han, C. (2014) Generation of male germ cells from mouse induced pluripotent stem cells in vitro, Stem Cell Res 12, 517-530. Gan, H., Wen, L., Liao, S., Lin, X., Ma, T., Liu, J., Song, C.-x., Wang, M., He, C., *Han, C., and *Tang, F. (2013) Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis，Nature Communications 4, 1995. Wang, F., Yang, Y., Lin, X., Wang, J. Q., Wu, Y. S., Xie, W., Wang, D., Zhu, S., Liao, Y. Q., Sun, Q., Yang, Y. G., *Guo, C., *Han, C., and *Tang, T. S. (2013) Genome-wide Loss of 5-hmC is a Novel Epigenetic Feature of Huntington's Disease, Human Molecular Genetics 22, 3641-3653. Wu, Y., Liao, S., Wang, X., Wang, S., Wang, M., and Han, C.* (2013) HSF2BP represses BNC1 transcriptional activity by sequestering BNC1 to the cytoplasm, FEBS Letters (10.1016/j.febslet.2013.04.049). Shi, Y., Zhuang, X., Xu, B., Hua, J., Liao, S., Shi, Q., Cooke, H. J., Han, C.* (2013) SYCP3-like X-linked 2 is expressed in meiotic germ cells and interacts with synaptonemal complex central element protein 2 and histone acetyltransferase TIP60, Gene（10.1016/j.gene.2013.06.033） Gan, H., Cai, T., Lin, X., Wu, Y., Wang, X., Yang, F., and Han, C.* (2013) Integrative Proteomic and Transcriptomic Analyses Reveal Multiple Post-transcriptional Regulatory Mechanisms of Mouse Spermatogenesis, Mol Cell Proteomics 12, 1144-1157. Hou, X., Zhang, W., Xiao, Z., Gan, H., Lin, X., Liao, S., and Han, C.* (2012) Mining and characterization of ubiquitin E3 ligases expressed in the mouse testis, BMC Genomics 13, 495. Zhang, Y., Wang, S., Wang, X., Liao, S., Wu, Y., and Han, C.* (2012) Endogenously produced FGF2 is essential for the survival and proliferation of cultured mouse spermatogonial stem cells, Cell Research 22, 773-776. Gan, H., Lin, X., Zhang, Z., Zhang, W., Liao, S., Wang, L., and Han, C.* (2011) piRNA profiling during specific stages of mouse spermatogenesis, RNA 17, 1191-1203. Zhuang, X. J., Hou, X. J., Liao, S. Y., Wang, X. X., Cooke, H. J., Zhang, M., and Han, C.* (2011) SLXL1, a novel acrosomal protein, interacts with DKKL1 and is involved in fertilization in mice, PLoS One 6, e20866. Shi, Y. Q., Liao, S. Y., Zhuang, X. J., and Han, C. S.* (2011) Mouse Fem1b interacts with and induces ubiquitin-mediated degradation of Ankrd37, Gene 485, 153-159. Liu, Z., Lin, X., Cai, Z., Zhang, Z., Han, C., Jia, S., Meng, A., and Wang, Q. (2011) Global identification of SMAD2 target genes reveals a role for multiple co-regulatory factors in zebrafish early gastrulas, The Journal of biological chemistry 286, 28520-28532. Liu, H., Wang, B., and Han, C.* (2011) Meta-analysis of genome-wide and replication association studies on prostate cancer, The Prostate 71, 209-224. Yang, Y., and Han, C.* (2010) GDNF stimulates the proliferation of cultured mouse immature Sertoli cells via its receptor subunit NCAM and ERK1/2 signaling pathway, BMC cell biology 11, 78. Shi, Y. Q., Li, Y. C., Hu, X. Q., Liu, T., Liao, S. Y., Guo, J., Huang, L., Hu, Z. Y., Tang, A. Y., Lee, K. F., Yeung, W. S., Han, C. S.*, and Liu, Y. X. (2009) Male germ cell-specific protein Trs4 binds to multiple proteins, BBRC 388, 583-588. Nian, H., Zhang, W., Shi, H., Zhao, Q., Xie, Q., Liao, S., Zhang, Y., Zhang, Z., Wang, C., and Han, C.* (2008) Mouse RING finger protein Rnf133 is a testis-specific endoplasmic reticulum-associated E3 ubiquitin ligase, Cell research 18, 800-802. Zhang, K., Shang, Y., Liao, S., Zhang, W., Nian, H., Liu, Y., Chen, Q., and Han, C.* (2007) Uncoupling protein 2 protects testicular germ cells from hyperthermia-induced apoptosis, BBRC 360, 327-332. Nian, H., Fan, C., Liao, S., Shi, Y., Zhang, K., Liu, Y., and Han, C.* (2007) RNF151, a testis-specific RING finger protein, interacts with dysbindin, Archives of biochemistry and biophysics 465, 157-163. Shi, Y. Q., Wang, Q. Z., Liao, S. Y., Zhang, Y., Liu, Y. X., and Han, C. S.* (2006) In vitro propagation of spermatogonial stem cells from KM mice, Front Biosci 11, 2614-2622. Walke, D. W., Han, C., Shaw, J., Wann, E., Zambrowicz, B., and Sands, A. (2001) In vivo drug target discovery: identifying the best targets from the genome, Current opinion in biotechnology 12, 626-631. Han, C. S.*, Chen, Y., Ezashi, T., and Roberts, R. M. (2001) Antiviral activities of the soluble extracellular domains of type I interferon receptors, PNAS 98, 6138-6143.