Research Interests:

Precise control of gene expression dosage is crucial for individual development. Gene expression levels are regulated by complex mechanisms at multiple levels. By deciphering the mechanisms that regulate gene expression dosage, we aim to achieve digital modeling of cells, which holds significant importance in understanding cellular functioning principles and guiding the development of artificial cells. Our research primarily focuses on two main directions: Investigation of the control mechanisms of gene expression dosage: In the mammalian genome, the majority of genes are expressed in a biallelic manner, while a small number of genes achieve precise monoallelic expression control through epigenetic mechanisms. We particularly emphasize two control mechanisms: imprinted genes on autosomes and X-chromosome inactivation in female cells. Using genomic analysis methods, we conduct research in the identification, functional and regulatory mechanism analysis, as well as model construction of monoallelically expressed genes. Artificial intelligence modeling of gene expression dosage: Leveraging massive multimodal omics data and integrating existing biological knowledge, we utilize various artificial intelligence tools to model gene expression dosage at different levels, including predicting the efficiency of regulatory elements, controlling the expression of individual genes, and capturing the interactions among multiple genes. Our ultimate goal is to achieve digital simulation of cells.

Awards and Honors:

Professional Activities:

Research Grants:

Selected Publications:

(# co-first author, * Corresponding author) 19: Yang X#, Liu G#, Feng G#, Bu D#, Wang P#, Jiang J#, Chen S#, Yang Q#, Miao H, Zhang Y, Man Z, Liang Z, Wang Z, Li Y, Li Z, Liu Y, Tian Y, Liu W, Li C, Li A, Dong J, Hu Z, Fang C, Cui L, Deng Z, Jiang H, Cui W, Zhang J, Yang Z, Li H, He X, Zhong L, Zhou J, Wang Z, Long Q, Xu P; X-Compass Consortium; Wang H, Meng Z, Wang X, Wang Y, Wang Y, Zhang S, Guo J, Zhao Y*, Zhou Y*, Li F*, Liu J*, Chen Y*, Yang G*, Li X*. GeneCompass: deciphering universal gene regulatory mechanisms with a knowledge-informed cross-species foundation model. Cell Res. 2024 Dec;34(12):830-845. 18: Li F#, Karimi N#, Wang S#, Pan T#, Dong J, Wang X, Ma S, Shan Q, Liu C, Zhang Y, Li W*, Feng G*. mRNA isoform switches during mouse zygotic genome activation. Cell Prolif. 2024 Jul;57(7):e13655. 17. Jiang L, Wang X, Wang L, Ma S, Ding Y, Liu C, Wang S, Shao X, Zhang Y, Li Z, Li W, Feng G*, Zhou Q*. Deciphering the placental abnormalities associated with somatic cell nuclear transfer at single-nucleus resolution. Protein Cell. 2023 Dec 1;14(12):924-928. 16. Wang Y, Yang N, Sun W, Zhao C, Hu X, Lu S, Cao S, Wang N, Hai T, Feng G*, An C*, Wang H*. Characterisation of X chromosome status of human extended pluripotent stem cells. Cell Prolif. 2023 May;56(5):e13468. 15. Fang Z, Tian Y, Sui C, Guo Y, Hu X, Lai Y, Liao Z, Li J, Feng G*, Jin L*, Qian K*. Single-Cell Transcriptomics of Proliferative Phase Endometrium: Systems Analysis of Cell-Cell Communication Network Using CellChat. Front Cell Dev Biol. 2022 Jul 22;10:919731. 14. Gu Z#, Guo J#, Zhai J#, Feng G#, Wang X, Gao Z, Li K, Ji S, Wang L, Xu Y, Chen X, Wang Y, Guo S, Yang M, Li L, Han H, Jiang L, Wen Y, Wang L, Hao J, Li W, Wang S, Wang H, Gu Q. A Uterus-Inspired Niche Drives Blastocyst Development to the Early Organogenesis. Adv Sci (Weinh). 2022 Oct;9(28):e2202282. 13. He ZQ#, Li YH#, Feng GH#, Yuan XW#, Lu ZB#, Dai M, Hu YP, Zhang Y, Zhou Q, Li W. Pharmacological Perturbation of Mechanical Contractility Enables Robust Transdifferentiation of Human Fibroblasts into Neurons. Adv Sci (Weinh). 2022 May;9(13):e2104682. 12. Wan H, Fu R, Tong M, Wang Y, Wang L, Wang S, Zhang Y, Li W, Wang XJ, Feng G*. Influence of feeder cells on transcriptomic analysis of pluripotent stem cells. Cell Prolif. 2022 Feb;55(2):e13189. 11. Zhu H, Sun H, Yu D, Li T, Hai T, Liu C, Zhang Y, Chen Y, Dai X, Li Z, Li W, Liu R*, Feng G*, Zhou Q*. Transcriptome and DNA Methylation Profiles of Mouse Fetus and Placenta Generated by Round Spermatid Injection. Front Cell Dev Biol. 2021 Mar 16;9:632183. 10. An C#, Feng G#, Zhang J, Cao S, Wang Y, Wang N, Lu F, Zhou Q, Wang H. Overcoming Autocrine FGF Signaling-Induced Heterogeneity in Naive Human ESCs Enables Modeling of Random X Chromosome Inactivation. Cell Stem Cell. 2020 Sep 3;27(3):482-497.e4. 9. Wang LY#, Li ZK#, Wang LB#, Liu C#, Sun XH#, Feng GH#, Wang JQ, Li YF, Qiao LY, Nie H, Jiang LY, Sun H, Xie YL, Ma SN, Wan HF, Lu FL, Li W, Zhou Q. Overcoming Intrinsic H3K27me3 Imprinting Barriers Improves Post-implantation Development after Somatic Cell Nuclear Transfer. Cell Stem Cell. 2020 Aug 6;27(2):315-325.e5. 8. Li J#, Wang C#, Feng G#, Zhang L, Chen G, Sun H, Wang J, Zhang Y, Zhou Q, Li W. Rbm14 maintains the integrity of genomic DNA during early mouse embryogenesis via mediating alternative splicing. Cell Prolif. 2020 Jan;53(1):e12724. 7. Wang J#, Wang L#, Feng G#, Wang Y#, Li Y, Li X, Liu C, Jiao G, Huang C, Shi J, Zhou T, Chen Q, Liu Z, Li W, Zhou Q. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell. 2018 Dec 13;175(7):1887-1901.e18. 6. Li ZK#, Wang LY#, Wang LB#, Feng GH#, Yuan XW#, Liu C, Xu K, Li YH, Wan HF, Zhang Y, Li YF, Li X, Li W, Zhou Q, Hu BY. Generation of Bimaternal and Bipaternal Mice from Hypomethylated Haploid ESCs with Imprinting Region Deletions. Cell Stem Cell. 2018 Nov 1;23(5):665-676.e4. 5. Zhang W#, Wan H#, Feng G#, Qu J#, Wang J, Jing Y, Ren R, Liu Z, Zhang L, Chen Z, Wang S, Zhao Y, Wang Z, Yuan Y, Zhou Q, Li W, Liu GH, Hu B. SIRT6 deficiency results in developmental retardation in cynomolgus monkeys. Nature. 2018 Aug;560(7720):661-665. 4. Li TD#, Feng GH#, Li YF#, Wang M#, Mao JJ, Wang JQ, Li X, Wang XP, Qu B, Wang LY, Zhang XX, Wan HF, Cui TT, Wan C, Liu L, Zhao XY, Hu BY, Li W, Zhou Q. Rat embryonic stem cells produce fertile offspring through tetraploid complementation. Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):11974-11979. 3. Xu K#, Yang Y#, Feng GH#, Sun BF#, Chen JQ#, Li YF, Chen YS, Zhang XX, Wang CX, Jiang LY, Liu C, Zhang ZY, Wang XJ, Zhou Q, Yang YG, Li W. Mettl3-mediated m6A regulates spermatogonial differentiation and meiosis initiation. Cell Res. 2017 Sep;27(9):1100-1114. 2. Feng G#, Tong M#, Xia B#, Luo GZ, Wang M, Xie D, Wan H, Zhang Y, Zhou Q, Wang XJ. Ubiquitously expressed genes participate in cell-specific functions via alternative promoter usage. EMBO Rep. 2016 Sep;17(9):1304-13. 1. Li Z#, Wan H#, Feng G#, Wang L#, He Z, Wang Y, Wang XJ, Li W, Zhou Q, Hu B. Birth of fertile bimaternal offspring following intracytoplasmic injection of parthenogenetic haploid embryonic stem cells. Cell Res. 2016 Jan;26(1):135-8. Complete article list: https://pubmed.ncbi.nlm.nih.gov/?term=feng+guihai&sort=date https://scholar.google.com/citations?user=hvVSbxYAAAAJ&hl=en