Abstract: Successful implantation depends oil active dialogue between the maternal endometrium and the implanting blastocysts that is well controlled by groups of regulators at the molecular level, Dickkopf2 (Dkk2) is a member of Dickkopf family normally acting as an antagonist of canonical Wnt/beta-catenin signaling, which has been proven to participate in tumorigenesis and early embryo development. In order to explore the potential function of Dkk2 in embryo implantation, the present study investigated the uterine expression and regulation profiles of Dkk2 during periimplantation in mice. Using reverse transcription-polymerase chain reaction, immunohistochemistry and Western blotting, we showed that the mRNA and protein levels of Dkk2 began to increase in the glandular epithelium on day 4, continued to increase on day 5 and then decreased from day 6 of pregnancy. Moreover, on days 5-8 of pregnancy, Dkk2 was increasingly expressed in the deciduum Of file uterus, especially around the implanting embryos. In addition upregulation of Dkk2 was also observed in uteri treated with estrogen (estradiol-17 beta) as well as in oil-induced artificial decidualization, indicating that the expression of Dkk2 could be induced by both steroid hormone (estrogen) and the process of decidualization. Furthermore, in the postimplantation uterus, the Dkk2 protein showed all inversed expression with active beta-catenin from day 6 onward, supporting the notion that Dkk2 plays an inhibitory role against canonical Writ signaling in the context of the decidualizing stroma. Collectively, our data suggests that Dkk2 expression is associated with uterine receptivity changes its well as the process of decidualization and that it might play important roles through inhibition of canonical Writ signaling in the periimplantation uterus.
Zhang Y , Peng S, Kuang HB, Chen Q, Liu S, Zhang L, Duan EK. 2009. Expression and Regulation of Dickkopf2 During Periimplantation in Mice. JOURNAL OF REPRODUCTION AND DEVELOPMENT. 55(1):17-22.
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